气候变化领域集成服务门户(CCPortal): Rapid hyperpolarization and purification of the metabolite fumarate in aqueous solution

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DOI	10.1073/pnas.2025383118
	Rapid hyperpolarization and purification of the metabolite fumarate in aqueous solution
	Knecht S.; Blanchard J.W.; Barskiy D.; Cavallari E.; Dagys L.; van Dyke E.; Tsukanov M.; Bliemel B.; Münnemann K.; Aime S.; Reineri F.; Levitt M.H.; Buntkowsky G.; Pines A.; Blümler P.; Budker D.; Eills J.
发表日期	2021
ISSN	00278424
卷号	118 期号:13
英文摘要	Hyperpolarized fumarate is a promising biosensor for carbon-13 magnetic resonance metabolic imaging. Such molecular imaging applications require nuclear hyperpolarization to attain sufficient signal strength. Dissolution dynamic nuclear polarization is the current state-of-the-art methodology for hyperpolarizing fumarate, but this is expensive and relatively slow. Alternatively, this important biomolecule can be hyperpolarized in a cheap and convenient manner using parahydrogen-induced polarization. However, this process requires a chemical reaction, and the resulting solutions are contaminated with the catalyst, unreacted reagents, and reaction side-product molecules, and are hence unsuitable for use in vivo. In this work we show that the hyperpolarized fumarate can be purified from these contaminants by acid precipitation as a pure solid, and later redissolved to a desired concentration in a clean aqueous solvent. Significant advances in the reaction conditions and reactor equipment allow for formation of hyperpolarized fumarate at 13C polarization levels of 30–45%. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Biomarker; Hyperpolarization; Metabolism; MRI; Parahydrogen
语种	英语
scopus关键词	carbon 13; fumaric acid derivative; hydrogen; solvent; acid precipitation; aqueous solution; Article; chemical reaction; concentration (parameter); controlled study; flow rate; hydrogenation; hyperpolarization; in vitro study; in vivo study; nuclear magnetic resonance imaging; osmolarity; physical chemistry; priority journal; purification; temperature measurement
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180102
作者单位	Eduard-Zintl-Institute for Inorganic Chemistry and Physical Chemistry, Technical University Darmstadt, Darmstadt, 64287, Germany; GSI Helmholtzzentrum für Schwerionenforschung GmbH, Helmholtz-Institut Mainz, Mainz, 55128, Germany; Department of Chemistry, University of California, Berkeley, CA 94720, United States; Department of Molecular Biotechnology and Health Sciences, Center of Molecular Imaging, University of Turin, Turin, 10126, Italy; Department of Chemistry, University of Southampton, Southampton, SO17 1BJ, United Kingdom; Department of Mechanical and Process Engineering, Technical University of Kaiserslautern, Kaiserslautern, 67663, Germany; Institute for Physics, Johannes Gutenberg University, Mainz, D-55090, Germany
推荐引用方式 GB/T 7714	Knecht S.,Blanchard J.W.,Barskiy D.,et al. Rapid hyperpolarization and purification of the metabolite fumarate in aqueous solution[J],2021,118(13).
APA	Knecht S..,Blanchard J.W..,Barskiy D..,Cavallari E..,Dagys L..,...&Eills J..(2021).Rapid hyperpolarization and purification of the metabolite fumarate in aqueous solution.Proceedings of the National Academy of Sciences of the United States of America,118(13).
MLA	Knecht S.,et al."Rapid hyperpolarization and purification of the metabolite fumarate in aqueous solution".Proceedings of the National Academy of Sciences of the United States of America 118.13(2021).