气候变化领域集成服务门户(CCPortal): Assembly of a dsRNA synthesizing complex: RNA-DEPENDENT RNA POLYMERASE 2 contacts the largest subunit of NUCLEAR RNA POLYMERASE IV

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DOI	10.1073/pnas.2019276118
	Assembly of a dsRNA synthesizing complex: RNA-DEPENDENT RNA POLYMERASE 2 contacts the largest subunit of NUCLEAR RNA POLYMERASE IV
	Mishra V.; Singh J.; Wang F.; Zhang Y.; Fukudome A.; Trinidad J.C.; Takagi Y.; Pikaard C.S.
发表日期	2021
ISSN	00278424
卷号	118 期号:13
英文摘要	In plants, transcription of selfish genetic elements such as transposons and DNA viruses is suppressed by RNA-directed DNA methylation. This process is guided by 24-nt short-interfering RNAs (siRNAs) whose double-stranded precursors are synthesized by DNA-dependent NUCLEAR RNA POLYMERASE IV (Pol IV) and RNA-DEPENDENT RNA POLYMERASE 2 (RDR2). Pol IV and RDR2 coimmunoprecipitate, and their activities are tightly coupled, yet the basis for their association is unknown. Here, we show that an interval near the RDR2 active site contacts the Pol IV catalytic subunit, NRPD1, the largest of Pol IV’s 12 subunits. Contacts between the catalytic regions of the two enzymes suggests that RDR2 is positioned to rapidly engage the free 3′ ends of Pol IV transcripts and convert these single-stranded transcripts into double-stranded RNAs (dsRNAs). © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Gene silencing; Pol IV; RDR2; RNA interference; RNA-directed DNA methylation
语种	英语
scopus关键词	double stranded RNA; RNA directed RNA polymerase; RNA polymerase II; RNA polymerase IV; RNA precursor; small interfering RNA; unclassified drug; Article; controlled study; DNA methylation; DNA virus; enzyme active site; nonhuman; priority journal; reverse transcription; RNA 3' end processing; RNA synthesis; RNA transcription; transposon
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180059
作者单位	HHMI, Indiana University, Bloomington, IN 47405, United States; Department of Biology, Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN 47405, United States; Department of Chemistry, Indiana University, Bloomington, IN 47405, United States; Laboratory for Biological Mass Spectrometry, Indiana University, Bloomington, IN 47405, United States; Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, United States
推荐引用方式 GB/T 7714	Mishra V.,Singh J.,Wang F.,et al. Assembly of a dsRNA synthesizing complex: RNA-DEPENDENT RNA POLYMERASE 2 contacts the largest subunit of NUCLEAR RNA POLYMERASE IV[J],2021,118(13).
APA	Mishra V..,Singh J..,Wang F..,Zhang Y..,Fukudome A..,...&Pikaard C.S..(2021).Assembly of a dsRNA synthesizing complex: RNA-DEPENDENT RNA POLYMERASE 2 contacts the largest subunit of NUCLEAR RNA POLYMERASE IV.Proceedings of the National Academy of Sciences of the United States of America,118(13).
MLA	Mishra V.,et al."Assembly of a dsRNA synthesizing complex: RNA-DEPENDENT RNA POLYMERASE 2 contacts the largest subunit of NUCLEAR RNA POLYMERASE IV".Proceedings of the National Academy of Sciences of the United States of America 118.13(2021).