气候变化领域集成服务门户(CCPortal): ZYP1 is required for obligate cross-over formation and cross-over interference in Arabidopsis

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DOI	10.1073/pnas.2021671118
	ZYP1 is required for obligate cross-over formation and cross-over interference in Arabidopsis
	France M.G.; Enderle J.; Röhrig S.; Puchta H.; Franklin F.C.H.; Higgins J.D.
发表日期	2021
ISSN	00278424
卷号	118 期号:14
英文摘要	The synaptonemal complex is a tripartite proteinaceous ultrastructure that forms between homologous chromosomes during prophase I of meiosis in the majority of eukaryotes. It is characterized by the coordinated installation of transverse filament proteins between two lateral elements and is required for wild-type levels of crossing over and meiotic progression. We have generated null mutants of the duplicated Arabidopsis transverse filament genes zyp1a and zyp1b using a combination of T-DNA insertional mutants and targeted CRISPR/Cas mutagenesis. Cytological and genetic analysis of the zyp1 null mutants reveals loss of the obligate chiasma, an increase in recombination map length by 1.3- to 1.7- fold and a virtual absence of cross-over (CO) interference, determined by a significant increase in the number of double COs. At diplotene, the numbers of HEI10 foci, a marker for Class I interference-sensitive COs, are twofold greater in the zyp1 mutant compared to wild type. The increase in recombination in zyp1 does not appear to be due to the Class II interference-insensitive COs as chiasmata were reduced by ∼52% in msh5/zyp1 compared to msh5. These data suggest that ZYP1 limits the formation of closely spaced Class I COs in Arabidopsis. Our data indicate that installation of ZYP1 occurs at ASY1-labeled axial bridges and that loss of the protein disrupts progressive coalignment of the chromosome axes. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cross-over interference; Meiosis; Recombination; Synaptonemal complex
语种	英语
scopus关键词	Arabidopsis; article; chromosome; controlled study; genetic analysis; genetic marker; loss of function mutation; meiotic prophase I; nonhuman; synaptonemal complex; wild type
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179968
作者单位	Department of Genetics and Genome Biology, University of Leicester, Leicester, LE1 7RH, United Kingdom; Botanical Institute Molecular Biology and Biochemistry, Karlsruhe Institute of Technology, Karlsruhe, 76131, Germany; School of Biosciences, University of Birmingham, Birmingham, B15 2TT, United Kingdom
推荐引用方式 GB/T 7714	France M.G.,Enderle J.,Röhrig S.,et al. ZYP1 is required for obligate cross-over formation and cross-over interference in Arabidopsis[J],2021,118(14).
APA	France M.G.,Enderle J.,Röhrig S.,Puchta H.,Franklin F.C.H.,&Higgins J.D..(2021).ZYP1 is required for obligate cross-over formation and cross-over interference in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America,118(14).
MLA	France M.G.,et al."ZYP1 is required for obligate cross-over formation and cross-over interference in Arabidopsis".Proceedings of the National Academy of Sciences of the United States of America 118.14(2021).