气候变化领域集成服务门户(CCPortal): Riboflavin instability is a key factor underlying the requirement of a gut microbiota for mosquito development

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DOI	10.1073/pnas.2101080118
	Riboflavin instability is a key factor underlying the requirement of a gut microbiota for mosquito development
	Wang Y.; Eum J.H.; Harrison R.E.; Valzania L.; Yang X.; Johnson J.A.; Huck D.T.; Brown M.R.; Strand M.R.
发表日期	2021
ISSN	00278424
卷号	118 期号:15
英文摘要	We previously determined that several diets used to rear Aedes aegypti and other mosquito species support the development of larvae with a gut microbiota but do not support the development of axenic larvae. In contrast, axenic larvae have been shown to develop when fed other diets. To understand the mechanisms underlying this dichotomy, we developed a defined diet that could be manipulated in concert with microbiota composition and environmental conditions. Initial studies showed that axenic larvae could not grow under standard rearing conditions (27 °C, 16-h light: 8-h dark photoperiod) when fed a defined diet but could develop when maintained in darkness. Downstream assays identified riboflavin decay to lumichrome as the key factor that prevented axenic larvae from growing under standard conditions, while gut community members like Escherichia coli rescued development by being able to synthesize riboflavin. Earlier results showed that conventional and gnotobiotic but not axenic larvae exhibit midgut hypoxia under standard rearing conditions, which correlated with activation of several pathways with essential growth functions. In this study, axenic larvae in darkness also exhibited midgut hypoxia and activation of growth signaling but rapidly shifted to midgut normoxia and arrested growth in light, which indicated that gut hypoxia was not due to aerobic respiration by the gut microbiota but did depend on riboflavin that only resident microbes could provide under standard conditions. Overall, our results identify riboflavin provisioning as an essential function for the gut microbiota under most conditions A. aegypti larvae experience in the laboratory and field. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Growth; Insect; Microbiota; Molting; Respiration
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179928
作者单位	Department of Entomology, University of Georgia, Athens, GA 30602, United States; Genetics and Developmental Biology Unit, Institut Curie, CNRS, UMR 321526, Paris, 75248, France
推荐引用方式 GB/T 7714	Wang Y.,Eum J.H.,Harrison R.E.,et al. Riboflavin instability is a key factor underlying the requirement of a gut microbiota for mosquito development[J],2021,118(15).
APA	Wang Y..,Eum J.H..,Harrison R.E..,Valzania L..,Yang X..,...&Strand M.R..(2021).Riboflavin instability is a key factor underlying the requirement of a gut microbiota for mosquito development.Proceedings of the National Academy of Sciences of the United States of America,118(15).
MLA	Wang Y.,et al."Riboflavin instability is a key factor underlying the requirement of a gut microbiota for mosquito development".Proceedings of the National Academy of Sciences of the United States of America 118.15(2021).