气候变化领域集成服务门户(CCPortal): An ABCC-type transporter endowing glyphosate resistance in plants

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DOI	10.1073/pnas.2100136118
	An ABCC-type transporter endowing glyphosate resistance in plants
	Pan L.; Yu Q.; Wang J.; Han H.; Mao L.; Nyporko A.; Maguza A.; Fan L.; Bai L.; Powles S.
发表日期	2021
ISSN	00278424
卷号	118 期号:16
英文摘要	Glyphosate is the most widely used herbicide in world agriculture and for general vegetation control in a wide range of situations. Global and often intensive glyphosate selection of very large weedy plant populations has resulted in widespread glyphosate resistance evolution in populations of many weed species. Here, working with a glyphosate-resistant (GR) Echinochloa colona population that evolved in a Western Australia agricultural field, we identified an ATP-binding cassette (ABC) transporter (EcABCC8) that is consistently up-regulated in GR plants. When expressed in transgenic rice, this EcABCC8 transporter endowed glyphosate resistance. Equally, rice, maize, and soybean overexpressing the EcABCC8 ortholog genes were made resistant to glyphosate. Conversely, CRISPR/Cas9-mediated knockout of the EcABCC8 ortholog gene OsABCC8 increased rice susceptibility to glyphosate. Subcellular localization analysis and quantification of glyphosate cellular levels in treated ABCC8 transgenic rice plants and isolated leaf protoplasts as well as structural modeling support that EcABCC8 is likely a plasma membrane-localized transporter extruding cytoplasmic glyphosate to the apoplast, lowering the cellular glyphosate level. This is a report of a membrane transporter effluxing glyphosate in a GR plant species, and its function is likely conserved in crop plant species. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	ABC transporter; Echinochloa colona; Glyphosate exporter; Glyphosate resistance; Plasma membrane
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179853
作者单位	Hunan Weed Science Key Laboratory, Hunan Academy of Agricultural Sciences, Changsha, 410125, China; College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China; Australian Herbicide Resistance Initiative, School of Agriculture and Environment, University of Western AustraliaWA 6009, Australia; Institute of Crop Science, Zhejiang University-Xuan Gu Agricultural Joint Innovation Center, Zhejiang University, Hangzhou, 310058, China; Department of Molecular Biotechnology and Bioinformatics, Taras Shevchenko National University of Kyiv, Kiev, 01033, Ukraine
推荐引用方式 GB/T 7714	Pan L.,Yu Q.,Wang J.,et al. An ABCC-type transporter endowing glyphosate resistance in plants[J],2021,118(16).
APA	Pan L..,Yu Q..,Wang J..,Han H..,Mao L..,...&Powles S..(2021).An ABCC-type transporter endowing glyphosate resistance in plants.Proceedings of the National Academy of Sciences of the United States of America,118(16).
MLA	Pan L.,et al."An ABCC-type transporter endowing glyphosate resistance in plants".Proceedings of the National Academy of Sciences of the United States of America 118.16(2021).