气候变化领域集成服务门户(CCPortal): A translational riboswitch coordinates nascent transcription-translation coupling

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DOI	10.1073/pnas.2023426118
	A translational riboswitch coordinates nascent transcription-translation coupling
	Chatterjee S.; Chauvier A.; Dandpat S.S.; Artsimovitch I.; Walter N.G.
发表日期	2021
ISSN	00278424
卷号	118 期号:16
英文摘要	Bacterial messenger RNA (mRNA) synthesis by RNA polymerase (RNAP) and first-round translation by the ribosome are often coupled to regulate gene expression, yet how coupling is established and maintained is ill understood. Here, we develop biochemical and single-molecule fluorescence approaches to probe the dynamics of RNAP-ribosome interactions on an mRNA with a translational preQ1sensing riboswitch in its 5′ untranslated region. Binding of preQ1 leads to the occlusion of the ribosome binding site (RBS), inhibiting translation initiation. We demonstrate that RNAP poised within the mRNA leader region promotes ribosomal 30S subunit binding, antagonizing preQ1-induced RBS occlusion, and that the RNAP-30S bridging transcription factors NusG and RfaH distinctly enhance 30S recruitment and retention, respectively. We further find that, while 30S-mRNA interaction significantly impedes RNAP in the absence of translation, an actively translating ribosome promotes productive transcription. A model emerges wherein mRNA structure and transcription factors coordinate to dynamically modulate the efficiency of transcription-translation coupling. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Nascent mRNA; Ribosome; RNA polymerase; Transcription-translation coupling; Translational riboswitch
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179825
作者单位	Single Molecule Analysis Group, Department of Chemistry, Center for RNA Biomedicine, University of Michigan, Ann Arbor, MI 48109, United States; Department of Microbiology, The Ohio State University, Columbus, OH 43210, United States
推荐引用方式 GB/T 7714	Chatterjee S.,Chauvier A.,Dandpat S.S.,et al. A translational riboswitch coordinates nascent transcription-translation coupling[J],2021,118(16).
APA	Chatterjee S.,Chauvier A.,Dandpat S.S.,Artsimovitch I.,&Walter N.G..(2021).A translational riboswitch coordinates nascent transcription-translation coupling.Proceedings of the National Academy of Sciences of the United States of America,118(16).
MLA	Chatterjee S.,et al."A translational riboswitch coordinates nascent transcription-translation coupling".Proceedings of the National Academy of Sciences of the United States of America 118.16(2021).