气候变化领域集成服务门户(CCPortal): Patterns of bacterial motility in microfluidics-confining environments

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DOI	10.1073/pnas.2013925118
	Patterns of bacterial motility in microfluidics-confining environments
	Tokárová V.; Perumal A.S.; Nayak M.; Shum H.; Kašpar O.; Rajendran K.; Mohammadi M.; Tremblay C.; Gaffney E.A.; Martel S.; Nicolau D.V.; Jr.; Nicolau D.V.
发表日期	2021
ISSN	00278424
卷号	118 期号:17
英文摘要	Understanding the motility behavior of bacteria in confining microenvironments, in which they search for available physical space and move in response to stimuli, is important for environmental, food industry, and biomedical applications. We studied the motility of five bacterial species with various sizes and flagellar architectures (Vibrio natriegens, Magnetococcus marinus, Pseudomonas putida, Vibrio fischeri, and Escherichia coli) in microfluidic environments presenting various levels of confinement and geometrical complexity, in the absence of external flow and concentration gradients. When the confinement is moderate, such as in quasi-open spaces with only one limiting wall, and in wide channels, the motility behavior of bacteria with complex flagellar architectures approximately follows the hydrodynamics-based predictions developed for simple monotrichous bacteria. Specifically, V. natriegens and V. fischeri moved parallel to the wall and P. putida and E. coli presented a stable movement parallel to the wall but with incidental wall escape events, while M. marinus exhibited frequent flipping between wall accumulator and wall escaper regimes. Conversely, in tighter confining environments, the motility is governed by the steric interactions between bacteria and the surrounding walls. In mesoscale regions, where the impacts of hydrodynamics and steric interactions overlap, these mechanisms can either push bacteria in the same directions in linear channels, leading to smooth bacterial movement, or they could be oppositional (e.g., in mesoscale-sized meandered channels), leading to chaotic movement and subsequent bacterial trapping. The study provides a methodological template for the design of microfluidic devices for single-cell genomic screening, bacterial entrapment for diagnostics, or biocomputation. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Bacterial motility; Microfluidic devices; Space partitioning; Wall accumulator; Wall escaper
语种	英语
scopus关键词	Aliivibrio fischeri; article; Escherichia coli; hydrodynamics; microfluidics; nonhuman; prediction; Pseudomonas putida
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179754
作者单位	Faculty of Engineering, Department of Bioengineering, McGill University, Montreal, QC H3A 0C3, Canada; Department of Chemical Engineering, University of Chemistry and Technology, Prague, Prague, 166 28, Czech Republic; Department of Applied Mathematics, University of Waterloo, Waterloo, ON N2L 3G1, Canada; Department of Computer Engineering, École Polytechnique de Montréal, Montreal, QC H3T 1J4, Canada; School of Mathematical Sciences, Queensland University of Technology, Brisbane, QLD 4000, Australia
推荐引用方式 GB/T 7714	Tokárová V.,Perumal A.S.,Nayak M.,et al. Patterns of bacterial motility in microfluidics-confining environments[J],2021,118(17).
APA	Tokárová V..,Perumal A.S..,Nayak M..,Shum H..,Kašpar O..,...&Nicolau D.V..(2021).Patterns of bacterial motility in microfluidics-confining environments.Proceedings of the National Academy of Sciences of the United States of America,118(17).
MLA	Tokárová V.,et al."Patterns of bacterial motility in microfluidics-confining environments".Proceedings of the National Academy of Sciences of the United States of America 118.17(2021).