气候变化领域集成服务门户(CCPortal): Structure and activity of SLAC1 channels for stomatal signaling in leaves

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DOI	10.1073/pnas.2015151118
	Structure and activity of SLAC1 channels for stomatal signaling in leaves
	Deng Y.-N.; Kashtoh H.; Wang Q.; Zhen G.-X.; Li Q.-Y.; Tang L.-H.; Gao H.-L.; Zhang C.-R.; Qin L.; Su M.; Li F.; Huang X.-H.; Wang Y.-C.; Xie Q.; Clarke O.B.; Hendrickson W.A.; Chen Y.-H.
发表日期	2021
ISSN	00278424
卷号	118 期号:18
英文摘要	Stomata in leaves regulate gas exchange between the plant and its atmosphere. Various environmental stimuli elicit abscisic acid (ABA); ABA leads to phosphoactivation of slow anion channel 1 (SLAC1); SLAC1 activity reduces turgor pressure in aperture-defining guard cells; and stomatal closure ensues. We used electrophysiology for functional characterizations of Arabidopsis thaliana SLAC1 (AtSLAC1) and cryoelectron microscopy (cryo-EM) for structural analysis of Brachypodium distachyon SLAC1 (BdSLAC1), at 2.97-Å resolution. We identified 14 phosphorylation sites in AtSLAC1 and showed nearly 330-fold channel-activity enhancement with 4 to 6 of these phosphorylated. Seven SLAC1-conserved arginines are poised in BdSLAC1 for regulatory interaction with the N-terminal extension. This BdSLAC1 structure has its pores closed, in a basal state, spring loaded by phenylalanyl residues in high-energy conformations. SLAC1 phosphorylation fine-tunes an equilibrium between basal and activated SLAC1 trimers, thereby controlling the degree of stomatal opening. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Abscisic acid signaling; Channel activation; Cryo-EM; Electrophysiology; Phosphorylation
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179690
作者单位	State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China; Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China; Department of Biology, New York University, New York, NY 10003, United States; Department of Physiology and Cellular Biophysics, Columbia University, New York, NY 10032, United States; Department of Anesthesiology, Columbia University, New York, NY 10032, United States; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, United States; CAS Center for Excellence in Biomacromolecules, Chinese Academy of Sciences, Beijing, 100101, China
推荐引用方式 GB/T 7714	Deng Y.-N.,Kashtoh H.,Wang Q.,et al. Structure and activity of SLAC1 channels for stomatal signaling in leaves[J],2021,118(18).
APA	Deng Y.-N..,Kashtoh H..,Wang Q..,Zhen G.-X..,Li Q.-Y..,...&Chen Y.-H..(2021).Structure and activity of SLAC1 channels for stomatal signaling in leaves.Proceedings of the National Academy of Sciences of the United States of America,118(18).
MLA	Deng Y.-N.,et al."Structure and activity of SLAC1 channels for stomatal signaling in leaves".Proceedings of the National Academy of Sciences of the United States of America 118.18(2021).