气候变化领域集成服务门户(CCPortal): DNA methylation mutants in Physcomitrella patens elucidate individual roles of CG and non-CG methylation in genome regulation

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DOI	10.1073/PNAS.2011361117
	DNA methylation mutants in Physcomitrella patens elucidate individual roles of CG and non-CG methylation in genome regulation
	Domb K.; Katz A.; Harris K.D.; Yaari R.; Kaisler E.; Nguyen V.H.; Hong U.V.T.; Griess O.; Heskiau K.G.; Ohad N.; Zemach A.
发表日期	2021
ISSN	00278424
起始页码	33700
结束页码	33710
卷号	117 期号:52
英文摘要	Cytosine (DNA) methylation in plants regulates the expression of genes and transposons. While methylation in plant genomes occurs at CG, CHG, and CHH sequence contexts, the comparative roles of the individual methylation contexts remain elusive. Here, we present Physcomitrella patens as the second plant system, besides Arabidopsis thaliana, with viable mutants with an essentially complete loss of methylation in the CG and non-CG contexts. In contrast to A. thaliana, P. patens has more robust CHH methylation, similar CG and CHG methylation levels, and minimal crosstalk between CG and non-CG methylation, making it possible to study context-specific effects independently. Our data found CHH methylation to act in redundancy with symmetric methylation in silencing transposons and to regulate the expression of CG/CHG-depleted transposons. Specific elimination of CG methylation did not dysregulate transposons or genes. In contrast, exclusive removal of non-CG methylation massively up-regulated transposons and genes. In addition, comparing two exclusively but equally CG- or CHG-methylated genomes, we show that CHG methylation acts as a greater transcriptional regulator than CG methylation. These results disentangle the transcriptional roles of CG and non-CG, as well as symmetric and asymmetric methylation in a plant genome, and point to the crucial role of non-CG methylation in genome regulation. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	CG methylation; CHG methylation; CHH methylation; Non-CG methylation; Transposons
语种	英语
scopus关键词	biological model; Bryopsida; DNA methylation; gene expression regulation; gene silencing; genetics; mutation; plant genome; transposon; upregulation; Bryopsida; DNA Methylation; DNA Transposable Elements; Epigenome; Gene Expression Regulation, Plant; Gene Silencing; Genome, Plant; Models, Genetic; Mutation; Up-Regulation
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179679
作者单位	School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, 69978, Israel
推荐引用方式 GB/T 7714	Domb K.,Katz A.,Harris K.D.,et al. DNA methylation mutants in Physcomitrella patens elucidate individual roles of CG and non-CG methylation in genome regulation[J],2021,117(52).
APA	Domb K..,Katz A..,Harris K.D..,Yaari R..,Kaisler E..,...&Zemach A..(2021).DNA methylation mutants in Physcomitrella patens elucidate individual roles of CG and non-CG methylation in genome regulation.Proceedings of the National Academy of Sciences of the United States of America,117(52).
MLA	Domb K.,et al."DNA methylation mutants in Physcomitrella patens elucidate individual roles of CG and non-CG methylation in genome regulation".Proceedings of the National Academy of Sciences of the United States of America 117.52(2021).