气候变化领域集成服务门户(CCPortal): Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse

CCPortal

DOI	10.1073/PNAS.2003991117
	Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse
	Li H.; Zhang Q.; Gu Y.; Wu Y.; Wang Y.; Wang L.; Feng S.; Hu Y.; Zheng Y.; Li Y.; Ye H.; Zhou B.; Lin L.; Liu M.; Yang H.; Li D.
发表日期	2021
ISSN	00278424
起始页码	33426
结束页码	33435
卷号	117 期号:52
英文摘要	Precise genetic engineering in specific cell types within an intact organism is intriguing yet challenging, especially in a spatiotemporal manner without the interference caused by chemical inducers. Here we engineered a photoactivatable Dre recombinase based on the identification of an optimal split site and demonstrated that it efficiently regulated transgene expression in mouse tissues spatiotemporally upon blue light illumination. Moreover, through a double-floxed inverted open reading frame strategy, we developed a Cre-activated light-inducible Dre (CALID) system. Taking advantage of well-defined cell-type–specific promoters or a well-established Cre transgenic mouse strain, we demonstrated that the CALID system was able to activate endogenous reporter expression for either bulk or sparse labeling of CaMKIIα-positive excitatory neurons and parvalbumin interneurons in the brain. This flexible and tunable system could be a powerful tool for the dissection and modulation of developmental and genetic complexity in a wide range of biological systems. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Cell labeling; Optogenetics; Photoactivatable Dre recombinase
语种	英语
scopus关键词	cre recombinase; Dre recombinase, E coli; Escherichia coli protein; integrase; recombinase; animal; brain; C57BL mouse; Dependoparvovirus; gene expression; gene vector; genetic engineering; genome; HEK293 cell line; human; light; liver; metabolism; nerve cell; reporter gene; time factor; transgenic mouse; Animals; Brain; Dependovirus; Escherichia coli Proteins; Gene Expression; Genes, Reporter; Genetic Engineering; Genetic Vectors; Genome; HEK293 Cells; Humans; Integrases; Light; Liver; Mice, Inbred C57BL; Mice, Transgenic; Neurons; Recombinases; Time Factors
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/179658
作者单位	Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai, 200241, China; Key Laboratory of Brain Functional Genomics (Ministry of Education), Institute of Brain Functional Genomics, East China Normal University, Shanghai, 200062, China; State Key Laboratory of Cell Biology, Chinese Academy of Sciences Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, China
推荐引用方式 GB/T 7714	Li H.,Zhang Q.,Gu Y.,et al. Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse[J],2021,117(52).
APA	Li H..,Zhang Q..,Gu Y..,Wu Y..,Wang Y..,...&Li D..(2021).Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse.Proceedings of the National Academy of Sciences of the United States of America,117(52).
MLA	Li H.,et al."Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse".Proceedings of the National Academy of Sciences of the United States of America 117.52(2021).