CCPortal
DOI10.1073/PNAS.2003991117
Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse
Li H.; Zhang Q.; Gu Y.; Wu Y.; Wang Y.; Wang L.; Feng S.; Hu Y.; Zheng Y.; Li Y.; Ye H.; Zhou B.; Lin L.; Liu M.; Yang H.; Li D.
发表日期2021
ISSN00278424
起始页码33426
结束页码33435
卷号117期号:52
英文摘要Precise genetic engineering in specific cell types within an intact organism is intriguing yet challenging, especially in a spatiotemporal manner without the interference caused by chemical inducers. Here we engineered a photoactivatable Dre recombinase based on the identification of an optimal split site and demonstrated that it efficiently regulated transgene expression in mouse tissues spatiotemporally upon blue light illumination. Moreover, through a double-floxed inverted open reading frame strategy, we developed a Cre-activated light-inducible Dre (CALID) system. Taking advantage of well-defined cell-type–specific promoters or a well-established Cre transgenic mouse strain, we demonstrated that the CALID system was able to activate endogenous reporter expression for either bulk or sparse labeling of CaMKIIα-positive excitatory neurons and parvalbumin interneurons in the brain. This flexible and tunable system could be a powerful tool for the dissection and modulation of developmental and genetic complexity in a wide range of biological systems. © 2020 National Academy of Sciences. All rights reserved.
英文关键词Cell labeling; Optogenetics; Photoactivatable Dre recombinase
语种英语
scopus关键词cre recombinase; Dre recombinase, E coli; Escherichia coli protein; integrase; recombinase; animal; brain; C57BL mouse; Dependoparvovirus; gene expression; gene vector; genetic engineering; genome; HEK293 cell line; human; light; liver; metabolism; nerve cell; reporter gene; time factor; transgenic mouse; Animals; Brain; Dependovirus; Escherichia coli Proteins; Gene Expression; Genes, Reporter; Genetic Engineering; Genetic Vectors; Genome; HEK293 Cells; Humans; Integrases; Light; Liver; Mice, Inbred C57BL; Mice, Transgenic; Neurons; Recombinases; Time Factors
来源期刊Proceedings of the National Academy of Sciences of the United States of America
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/179658
作者单位Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai, 200241, China; Key Laboratory of Brain Functional Genomics (Ministry of Education), Institute of Brain Functional Genomics, East China Normal University, Shanghai, 200062, China; State Key Laboratory of Cell Biology, Chinese Academy of Sciences Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, China
推荐引用方式
GB/T 7714
Li H.,Zhang Q.,Gu Y.,et al. Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse[J],2021,117(52).
APA Li H..,Zhang Q..,Gu Y..,Wu Y..,Wang Y..,...&Li D..(2021).Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse.Proceedings of the National Academy of Sciences of the United States of America,117(52).
MLA Li H.,et al."Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse".Proceedings of the National Academy of Sciences of the United States of America 117.52(2021).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li H.]的文章
[Zhang Q.]的文章
[Gu Y.]的文章
百度学术
百度学术中相似的文章
[Li H.]的文章
[Zhang Q.]的文章
[Gu Y.]的文章
必应学术
必应学术中相似的文章
[Li H.]的文章
[Zhang Q.]的文章
[Gu Y.]的文章
相关权益政策
暂无数据
收藏/分享

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。