气候变化领域集成服务门户(CCPortal): “Candidatus Ethanoperedens;” a Thermophilic Genus of Archaea Mediating the Anaerobic Oxidation of Ethane

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DOI	10.1128/mBio.00600-20
	“Candidatus Ethanoperedens;” a Thermophilic Genus of Archaea Mediating the Anaerobic Oxidation of Ethane
	Hahn C.J.; Laso-Pérez R.; Vulcano F.; Vaziourakis K.-M.; Stokke R.; Steen I.H.; Teske A.; Boetius A.; Liebeke M.; Amann R.; Knittel K.; Wegener G.
发表日期	2020
ISSN	21612129
卷号	11 期号:2
英文摘要	Cold seeps and hydrothermal vents deliver large amounts of methane and other gaseous alkanes into marine surface sediments. Consortia of archaea and partner bacteria thrive on the oxidation of these alkanes and its coupling to sulfate reduction. The inherently slow growth of the involved organisms and the lack of pure cultures have impeded the understanding of the molecular mechanisms of ar-chaeal alkane degradation. Here, using hydrothermal sediments of the Guaymas Basin (Gulf of California) and ethane as the substrate, we cultured microbial consortia of a novel anaerobic ethane oxidizer, “Candidatus Ethanoperedens thermophilum” (GoM-Arc1 clade), and its partner bacterium “Candidatus Desulfofervidus auxilii,” previously known from methane-oxidizing consortia. The sulfate reduction activity of the culture doubled within one week, indicating a much faster growth than in any other alkane-oxidizing archaea described before. The dominance of a single archaeal phylotype in this culture allowed retrieval of a closed genome of “Ca. Ethanopere-dens,” a sister genus of the recently reported ethane oxidizer “Candidatus Argoar-chaeum.” The metagenome-assembled genome of “Ca. Ethanoperedens” encoded a complete methanogenesis pathway including a methyl-coenzyme M reductase (MCR) that is highly divergent from those of methanogens and methanotrophs. Combined substrate and metabolite analysis showed ethane as the sole growth substrate and production of ethyl-coenzyme M as the activation product. Stable isotope probing demonstrated that the enzymatic mechanism of ethane oxidation in “Ca. Ethanope-redens” is fully reversible; thus, its enzymatic machinery has potential for the bio-technological development of microbial ethane production from carbon dioxide. IMPORTANCE In the seabed, gaseous alkanes are oxidized by syntrophic microbial consortia that thereby reduce fluxes of these compounds into the water column. Because of the immense quantities of seabed alkane fluxes, these consortia are key catalysts of the global carbon cycle. Due to their obligate syntrophic lifestyle, the physiology of alkane-degrading archaea remains poorly understood. We have now cultivated a thermophilic, relatively fast-growing ethane oxidizer in partnership with a sulfate-reducing bacterium known to aid in methane oxidation and have retrieved the first complete genome of a short-chain alkane-degrading archaeon. This will greatly enhance the understanding of nonmethane alkane activation by noncanoni-cal methyl-coenzyme M reductase enzymes and provide insights into additional metabolic steps and the mechanisms underlying syntrophic partnerships. Ultimately, this knowledge could lead to the biotechnological development of alkanogenic microorganisms to support the carbon neutrality of industrial processes. © 2020 Hahn et al.
英文关键词	Alkane degradation; Archaea; Hydrothermal vents; Methyl-coenzyme M reductase; Model organism; Syntrophy
scopus关键词	ethane; biological marker; ethane; RNA 16S; amino acid sequence; anaerobic metabolism; Article; bacterial genome; bacterial growth; Candidatus ethanoperedens; controlled study; enzyme kinetics; genetic code; metabolite; metagenome; molecular phylogeny; nonhuman; priority journal; species composition; stoichiometry; thermophilic archaeon; anaerobic growth; archaeal genome; archaeon; bacterium; classification; energy metabolism; genetics; genomics; hydrothermal vent; metabolism; microbiology; molecular typing; oxidation reduction reaction; phylogeny; procedures; sediment; Anaerobiosis; Archaea; Bacteria; Biomarkers; Energy Metabolism; Ethane; Genome, Archaeal; Genomics; Geologic Sediments; Hydrothermal Vents; Metabolic Networks and Pathways; Molecular Typing; Oxidation-Reduction; Phylogeny; RNA, Ribosomal, 16S
来源期刊	mBio
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/176669
作者单位	Max-Planck Institute for Marine Microbiology, Bremen, Germany; MARUM, Center for Marine Environmental Sciences, University of Bremen, Bremen, Germany; Alfred Wegener Institute Helmholtz Center for Polar and Marine Research, Bremerhaven, Germany; K.G. Jebsen Centre for Deep Sea Research and Department of Biological Sciences, University of Bergen, Bergen, Norway; University of Patras, Patras, Greece; The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States
推荐引用方式 GB/T 7714	Hahn C.J.,Laso-Pérez R.,Vulcano F.,等. “Candidatus Ethanoperedens;” a Thermophilic Genus of Archaea Mediating the Anaerobic Oxidation of Ethane[J],2020,11(2).
APA	Hahn C.J..,Laso-Pérez R..,Vulcano F..,Vaziourakis K.-M..,Stokke R..,...&Wegener G..(2020).“Candidatus Ethanoperedens;” a Thermophilic Genus of Archaea Mediating the Anaerobic Oxidation of Ethane.mBio,11(2).
MLA	Hahn C.J.,et al."“Candidatus Ethanoperedens;” a Thermophilic Genus of Archaea Mediating the Anaerobic Oxidation of Ethane".mBio 11.2(2020).