气候变化领域集成服务门户(CCPortal): Binding of Clitoria ternatea L. flower extract with α-amylase simultaneously monitored at two wavelengths using a photon streaming time-resolved fluorescence approach

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DOI	10.1016/j.saa.2018.11.062
	Binding of Clitoria ternatea L. flower extract with α-amylase simultaneously monitored at two wavelengths using a photon streaming time-resolved fluorescence approach
	Hungerford G.; Lemos M.A.; Chu B.-S.
发表日期	2019
ISSN	13861425
起始页码	108
结束页码	113
卷号	211
英文摘要	The binding of an extract from the flowers of Clitoria ternatea L. to the digestive enzyme α-amylase was investigated. This extract is a mixture of flavonoids, including anthocyanins, and has been previously shown to inhibit the activity this enzyme. This has implications for modulating starch digestion. In order to investigate the kinetics, we made use of time-resolved fluorescence to simultaneously monitor two different emission bands emanating from the extract. This measurement was enabled by the use of a “photon streaming” approach and changes in fluorescence lifetime and intensity were used to follow the interaction. A longer wavelength band (655 nm) was ascribed to anthocyanins in the mixture and these were observed to bind at a rate an order of magnitude slower than other flavonoids present in the extract, monitored at a shorter wavelength (485 nm). Changes in the fluorescence emission of the extract upon binding were further assessed by the use of decay associated spectra. © 2018 Elsevier B.V.
英文关键词	Anthocyanins; Butterfly pea; Enzyme inhibition; Flavonoids; TCSPC
scopus关键词	Anthocyanins; Enzyme activity; Enzyme inhibition; Flavonoids; Fluorescence; Mixtures; Photons; Butterfly pea; Clitoria ternatea; Digestive enzymes; Fluorescence emission; Fluorescence lifetimes; Shorter wavelength; TCSPC; Time-resolved fluorescence; Amylases; amylase; anthocyanin; flavonoid; plant extract; starch; antagonists and inhibitors; chemistry; Clitoria; flower; kinetics; metabolism; photon; procedures; spectrofluorometry; alpha-Amylases; Anthocyanins; Clitoria; Flavonoids; Flowers; Kinetics; Photons; Plant Extracts; Spectrometry, Fluorescence; Starch
来源期刊	Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/176465
作者单位	HORIBA Scientific, 133 Finnieston Street, Glasgow, G3 8HB, United Kingdom; Division of Food and Drink, School of Science, Engineering and Technology, Abertay University, 40 Bell Street, Dundee, DD1 1HG, United Kingdom
推荐引用方式 GB/T 7714	Hungerford G.,Lemos M.A.,Chu B.-S.. Binding of Clitoria ternatea L. flower extract with α-amylase simultaneously monitored at two wavelengths using a photon streaming time-resolved fluorescence approach[J],2019,211.
APA	Hungerford G.,Lemos M.A.,&Chu B.-S..(2019).Binding of Clitoria ternatea L. flower extract with α-amylase simultaneously monitored at two wavelengths using a photon streaming time-resolved fluorescence approach.Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy,211.
MLA	Hungerford G.,et al."Binding of Clitoria ternatea L. flower extract with α-amylase simultaneously monitored at two wavelengths using a photon streaming time-resolved fluorescence approach".Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy 211(2019).