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DOI	10.1016/j.still.2019.104302
	Bacterial and fungal diversity in rhizosphere and bulk soil under different long-term tillage and cereal/legume rotation
	Essel E.; Xie J.; Deng C.; Peng Z.; Wang J.; Shen J.; Xie J.; Coulter J.A.; Li L.
发表日期	2019
ISSN	1671987
卷号	194
英文摘要	Soil microbial diversity is important in maintaining soil quality, sustainable agriculture, and ecosystem function. Soil management practices can influence the diversity and activity of soil microbes in agricultural fields. The objective of this study was to assess the effects of tillage and stubble management on the diversity of bacteria and fungi, chemical property and total carbon emission in the rhizosphere and bulk soils in wheat (Triticum aestivum L.) -pea (Pisum arvense L.) rotation at pre-harvest. Treatments included conventional tillage with stubble removed (T), no-tillage with stubble removed (NT), conventional tillage with stubble incorporated (TS), and no-tillage with stubble retained (NTS). Bacteria 16S rRNA (V3V4) and fungi ITS (ITS2) region genes were sequenced from bulk soil and rhizosphere soils. Abundance of the dominant bacterial (Actinobacteria, Proteobacteria, Chloroflexi, Acidobacteria, and Planctomycetes) and fungal (Ascomycota and Basidiomycota) phyla identified did not differ significantly (P < 0.05) among treatments. Rhizobium and rare fungi Kurtzmanomyces occurred in the rhizosphere but were virtually absent in bulk soil. However, bacterial and fungal OTUs diversity indices were less in the rhizosphere compared to bulk soil. Soil from the NTS and NT had the greatest bacteria 16S rRNA and fungi ITS region number of OTUs; however, the microbial community did not differ among treatments. The abundance of class level bacteria and fungi were associated with soil pH, total organic carbon, total nitrogen, NH4 + -N, and NO3 ¯ -N contents. The results indicate that bulk soil had higher microbial library, which will be beneficial for establishment of the next season's microbial community. Elsevier B.V.
英文关键词	16S rRNA; ITS region; Microbial diversity; Stubble; Tillage
scopus关键词	Agronomy; Bacteria; Fungi; Organic carbon; RNA; 16S rRNA; ITS region; Microbial diversity; Stubble; Tillage; Soils; bacterium; cereal; crop rotation; fungus; legume; long-term change; microbial community; rhizosphere; RNA; soil microorganism; species diversity; tillage; Acidobacteria; Actinobacteria; Ascomycota; Bacteria (microorganisms); Basidiomycota; Chloroflexi; Chloroflexi (class); Fungi; Kurtzmanomyces; Otus; Pisum sativum; Planctomycetes; Proteobacteria; Rhizobium; Triticum aestivum
来源期刊	Soil and Tillage Research
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/176314
作者单位	Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou, China; College of Agronomy, Gansu Agricultural University, Lanzhou, China; Department of Applied Biology, University for Development Studies, Tamale, Ghana; Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55108, United States
推荐引用方式 GB/T 7714	Essel E.,Xie J.,Deng C.,et al. Bacterial and fungal diversity in rhizosphere and bulk soil under different long-term tillage and cereal/legume rotation[J],2019,194.
APA	Essel E..,Xie J..,Deng C..,Peng Z..,Wang J..,...&Li L..(2019).Bacterial and fungal diversity in rhizosphere and bulk soil under different long-term tillage and cereal/legume rotation.Soil and Tillage Research,194.
MLA	Essel E.,et al."Bacterial and fungal diversity in rhizosphere and bulk soil under different long-term tillage and cereal/legume rotation".Soil and Tillage Research 194(2019).