气候变化领域集成服务门户(CCPortal): A sustainable; high-performance process for the economic production of waste-free microbial oils that can replace plant-based equivalents

CCPortal

DOI	10.1039/c9ee00210c
	A sustainable; high-performance process for the economic production of waste-free microbial oils that can replace plant-based equivalents
	Masri M.A.; Garbe D.; Mehlmer N.; Brück T.B.
发表日期	2019
ISSN	1754-5692
起始页码	2717
结束页码	2732
卷号	12 期号:9
英文摘要	Globally, biofuel and oleochemical production based on plant oils negatively affects biodiversity. As an alternative triglyceride source, lipid production from oleaginous yeasts faces numerous challenges in feedstock availability, lipid productivity, downstream processing, and waste treatment, prohibiting the design of a cost-competitive process with regard to plant equivalents. In this study, we present a fully integrated operation for microbial oil production, which consolidates upstream and downstream processing with side-stream recycling. Co-fermentation of sugar and acetic acid was successfully implemented in fed-batch, semi-continuous, and continuous fermentation modes. Process validation was conducted at a 25 L scale with a lipid productivity of 1.2 g L-1 h-1. Cutaneotrichosporon oleaginosus cell debris was used as an inducer in Trichoderma reesei fermentation for on-site generation of yeast-specific cell-wall hydrolases. In situ hydrolase application allowed for efficient C. oleaginosus cell lysis (85% w/w) and simultaneous lipid release. A subsequent centrifugation step yielded 90% (w/w) recovery of intracellular lipids without the need for any organic solvent. The nutrient-rich water phase was applied as an internal sugar source for subsequent yeast fermentation cycles. With this yeast hydrolysate, the lipid productivity was considerably increased to 2.4 g L-1 h-1. A techno-economic analysis of the current lipid production processes estimated costs at $1.6 per kg lipid. Moreover, life cycle assessment analysis indicated an emission of 3.56 kg CO2 equivalents for every 1 kg produced yeast oil. Accordingly, we established an integrated operation for bioconversion of acetic acid and sugar to sustainable lipids at maximum productivity coupled with minimal waste generation and energy consumption. © 2019 The Royal Society of Chemistry.
语种	英语
scopus关键词	Acetic acid; Biodiversity; Cost benefit analysis; Energy utilization; Fermentation; Hydrolases; Life cycle; Lipids; pH; Productivity; Rivers; Waste treatment; Yeast; Continuous fermentation; Downstream-processing; Economic production; Integrated Operations; Life cycle assessment analysis; Lipid productivities; Maximum productivity; Techno-Economic analysis; Economic analysis; acetic acid; biodiversity; biofuel; essential oil; fermentation; life cycle analysis; solvent; sugar; Hypocrea jecorina
来源期刊	Energy and Environmental Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/162669
作者单位	Department of Chemistry, Werner Siemens Department of Synthetic Biotechnology, Technical University of Munich, Germany
推荐引用方式 GB/T 7714	Masri M.A.,Garbe D.,Mehlmer N.,et al. A sustainable; high-performance process for the economic production of waste-free microbial oils that can replace plant-based equivalents[J],2019,12(9).
APA	Masri M.A.,Garbe D.,Mehlmer N.,&Brück T.B..(2019).A sustainable; high-performance process for the economic production of waste-free microbial oils that can replace plant-based equivalents.Energy and Environmental Science,12(9).
MLA	Masri M.A.,et al."A sustainable; high-performance process for the economic production of waste-free microbial oils that can replace plant-based equivalents".Energy and Environmental Science 12.9(2019).