气候变化领域集成服务门户(CCPortal): Pathogen manipulation of chloroplast function triggers a light-dependent immune recognition

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DOI	10.1073/pnas.2002759117
	Pathogen manipulation of chloroplast function triggers a light-dependent immune recognition
	Gao C.; Xu H.; Huang J.; Sun B.; Zhang F.; Savage Z.; Duggan C.; Yan T.; Wu C.-H.; Wang Y.; Vleeshouwers V.G.A.A.; Kamoun S.; Bozkurt T.O.; Dong S.
发表日期	2020
ISSN	0027-8424
起始页码	9613
结束页码	9620
卷号	117 期号:17
英文摘要	In plants and animals, nucleotide-binding leucine-rich repeat (NLR) proteins are intracellular immune sensors that recognize and eliminate a wide range of invading pathogens. NLR-mediated immunity is known to be modulated by environmental factors. However, how pathogen recognition by NLRs is influenced by environmental factors such as light remains unclear. Here, we show that the agronomically important NLR Rpi-vnt1.1 requires light to confer disease resistance against races of the Irish potato famine pathogen Phytophthora infestans that secrete the effector protein AVRvnt1. The activation of Rpi-vnt1.1 requires a nuclear-encoded chloroplast protein, glycerate 3-kinase (GLYK), implicated in energy production. The pathogen effector AVRvnt1 binds the full-length chloroplast-targeted GLYK isoform leading to activation of Rpi-vnt1.1. In the dark, Rpi-vnt1.1-mediated resistance is compromised because plants produce a shorter GLYK-lacking the intact chloroplast transit peptide-that is not bound by AVRvnt1. The transition between full-length and shorter plant GLYK transcripts is controlled by a light-dependent alternative promoter selection mechanism. In plants that lack Rpi-vnt1.1, the presence of AVRvnt1 reduces GLYK accumulation in chloroplasts counteracting GLYK contribution to basal immunity. Our findings revealed that pathogen manipulation of chloroplast functions has resulted in a light-dependent immune response. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Chloroplast; Disease resistance; Light; NLR; Potato blight
语种	英语
scopus关键词	glycerate 3 kinase; phosphotransferase; protein; protein AVRvnt1; unclassified drug; fungal protein; glycerate kinase; nucleotide binding oligomerization domain like receptor; phosphotransferase; plant protein; Agrobacterium; antigen recognition; Article; chloroplast; confocal microscopy; controlled study; disease resistance; energy yield; gene silencing; immune response; immunoprecipitation; in vivo study; light; nonhuman; Phytophthora infestans; plant immunity; plasmid; priority journal; promoter region; protein binding; protein secretion; reverse transcription polymerase chain reaction; separation technique; Western blotting; animal; chloroplast; Escherichia coli; gene expression regulation; genetics; immunology; light; metabolism; microbiology; Phytophthora infestans; potato; radiation response; seedling; tobacco; two hybrid system; Agrobacterium; Animals; Chloroplasts; Escherichia coli; Fungal Proteins; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Gene Silencing; Light; Microscopy, Confocal; NLR Proteins; Phosphotransferases (Alcohol Group Acceptor); Phytophthora infestans; Plant Proteins; Seedlings; Solanum tuberosum; Tobacco; Two-Hybrid System Techniques
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160971
作者单位	Gao, C., College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China; Xu, H., College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China; Huang, J., College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China; Sun, B., College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China; Zhang, F., College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China; Savage, Z., Imperial College, South Kensington Campus, London, SW7 2AZ, United Kingdom; Duggan, C., Imperial College, South Kensington Campus, London, SW7 2AZ, United Kingdom; Yan, T., College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China; Wu, C.-H., Sainsbury Laboratory, University of East Anglia, Norwich, NR4 7UH, United Kingdom; Wang, Y., College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China, Key Laboratory of Plant Immunity, Nanjing Agricultural University, Nanjing, 21009...
推荐引用方式 GB/T 7714	Gao C.,Xu H.,Huang J.,et al. Pathogen manipulation of chloroplast function triggers a light-dependent immune recognition[J],2020,117(17).
APA	Gao C..,Xu H..,Huang J..,Sun B..,Zhang F..,...&Dong S..(2020).Pathogen manipulation of chloroplast function triggers a light-dependent immune recognition.Proceedings of the National Academy of Sciences of the United States of America,117(17).
MLA	Gao C.,et al."Pathogen manipulation of chloroplast function triggers a light-dependent immune recognition".Proceedings of the National Academy of Sciences of the United States of America 117.17(2020).