气候变化领域集成服务门户(CCPortal): A multiomic analysis of in situ coral-turf algal interactions

CCPortal

DOI	10.1073/pnas.1915455117
	A multiomic analysis of in situ coral-turf algal interactions
	Roach T.N.F.; Little M.; Arts M.G.I.; Huckeba J.; Haas A.F.; George E.E.; Quinn R.A.; Cobián-Güemes A.G.; Naliboff D.S.; Silveira C.B.; Vermeij M.J.A.; Kelly L.W.; Dorrestein P.C.; Rohwer F.
发表日期	2020
ISSN	0027-8424
起始页码	13588
结束页码	13595
卷号	117 期号:24
英文摘要	Viruses, microbes, and host macroorganisms form ecological units called holobionts. Here, a combination of metagenomic sequencing, metabolomic profiling, and epifluorescence microscopy was used to investigate how the different components of the holobiont including bacteria, viruses, and their associated metabolites mediate ecological interactions between corals and turf algae. The data demonstrate that there was a microbial assemblage unique to the coral-turf algae interface displaying higher microbial abundances and larger microbial cells. This was consistent with previous studies showing that turf algae exudates feed interface and coral-associated microbial communities, often at the detriment of the coral. Further supporting this hypothesis, when the metabolites were assigned a nominal oxidation state of carbon (NOSC), we found that the turf algal metabolites were significantly more reduced (i.e., have higher potential energy) compared to the corals and interfaces. The algae feeding hypothesis was further supported when the ecological outcomes of interactions (e.g., whether coral was winning or losing) were considered. For example, coral holobionts losing the competition with turf algae had higher Bacteroidetes-to-Firmicutes ratios and an elevated abundance of genes involved in bacterial growth and division. These changes were similar to trends observed in the obese human gut microbiome, where overfeeding of the microbiome creates a dysbiosis detrimental to the long-term health of the metazoan host. Together these results show that there are specific biogeochemical changes at coral-turf algal interfaces that predict the competitive outcomes between holobionts and are consistent with algal exudates feeding coral-associated microbes. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Coral reefs; Holobiont; Metabolomics; Metagenomics; Microbial ecology
语种	英语
scopus关键词	carbon; alga; Article; bacterial gene; bacterial growth; Bacteroidetes; coral; dysbiosis; energy resource; epifluorescence microscopy; Firmicutes; intestine flora; metabolic fingerprinting; microbial community; multiomics; nonhuman; organismal interaction; oxidation reduction state; priority journal; sequence analysis; turf algae; animal; Anthozoa; bacterium; chemistry; classification; coral reef; ecosystem; genetics; green alga; isolation and purification; metabolism; metagenomics; microbiology; microflora; parasitology; Animals; Anthozoa; Bacteria; Chlorophyta; Coral Reefs; Ecosystem; Metagenomics; Microbiota
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160916
作者单位	Roach, T.N.F., Hawai'i Institute of Marine Biology, University of Hawai'i at Manoa, Kane'ohe, HI 96744, United States, Biosphere 2, University of Arizona, Oracle, AZ 85739, United States, Department of Biology, San Diego State University, San Diego, CA 92182, United States, Viral Information Institute, San Diego State University, San Diego, CA 92182, United States; Little, M., Department of Biology, San Diego State University, San Diego, CA 92182, United States, Viral Information Institute, San Diego State University, San Diego, CA 92182, United States; Arts, M.G.I., Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, 1090 GE, Netherlands, Royal Netherlands Institute for Sea Research (NIOZ), Utrecht University, Den Burg, Texel, 1790 AB, Netherlands, Department of Freshwater and Marine Ecology, Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, 1090 GE, Netherlands; Huckeba, J., Institute for Biodiversity and Ecosystem Dynamics, U...
推荐引用方式 GB/T 7714	Roach T.N.F.,Little M.,Arts M.G.I.,et al. A multiomic analysis of in situ coral-turf algal interactions[J],2020,117(24).
APA	Roach T.N.F..,Little M..,Arts M.G.I..,Huckeba J..,Haas A.F..,...&Rohwer F..(2020).A multiomic analysis of in situ coral-turf algal interactions.Proceedings of the National Academy of Sciences of the United States of America,117(24).
MLA	Roach T.N.F.,et al."A multiomic analysis of in situ coral-turf algal interactions".Proceedings of the National Academy of Sciences of the United States of America 117.24(2020).