气候变化领域集成服务门户(CCPortal): Cofactor-enabled functional expression of fruit fly; honeybee; and bumblebee nicotinic receptors reveals picomolar neonicotinoid actions

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DOI	10.1073/pnas.2003667117
	Cofactor-enabled functional expression of fruit fly; honeybee; and bumblebee nicotinic receptors reveals picomolar neonicotinoid actions
	Ihara M.; Furutani S.; Shigetou S.; Shimada S.; Niki K.; Komori Y.; Kamiya M.; Koizumi W.; Magara L.; Hikida M.; Noguchi A.; Okuhara D.; Yoshinari Y.; Kondo S.; Tanimoto H.; Niwa R.; Sattelle D.B.; Matsuda K.
发表日期	2020
ISSN	0027-8424
起始页码	16283
结束页码	16291
卷号	117 期号:28
英文摘要	The difficulty of achieving robust functional expression of insect nicotinic acetylcholine receptors (nAChRs) has hampered our understanding of these important molecular targets of globally deployed neonicotinoid insecticides at a time when concerns have grown regarding the toxicity of this chemotype to insect pollinators. We show that thioredoxin-related transmembrane protein 3 (TMX3) is essential to enable robust expression in Xenopus laevis oocytes of honeybee (Apis mellifera) and bumblebee (Bombus terrestris) as well as fruit fly (Drosophila melanogaster) nAChR heteromers targeted by neonicotinoids and not hitherto robustly expressed. This has enabled the characterization of picomolar target site actions of neonicotinoids, findings important in understanding their toxicity. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Bumblebee; Fruit fly; Honeybee; Neonicotinoids; Nicotinic acetylcholine receptors
语种	英语
scopus关键词	clothianidin; imidacloprid; membrane protein; neonicotinoid; nicotinic receptor; thiacloprid; thioredoxin related transmembrane protein 3; unclassified drug; acetylcholine; insect protein; insecticide; neonicotinoid; nicotinic agent; nicotinic receptor; thioredoxin; Apis mellifera; Article; Bombus terrestris; controlled study; Drosophila melanogaster; nonhuman; oocyte; priority journal; protein expression; protein function; Xenopus laevis; animal; bee; dose response; genetics; metabolism; protein subunit; Acetylcholine; Animals; Bees; Dose-Response Relationship, Drug; Drosophila melanogaster; Insect Proteins; Insecticides; Neonicotinoids; Nicotinic Agonists; Oocytes; Protein Subunits; Receptors, Nicotinic; Thioredoxins; Xenopus laevis
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160889
作者单位	Ihara, M., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Furutani, S., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Shigetou, S., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Shimada, S., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Niki, K., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Komori, Y., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Kamiya, M., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Koizumi, W., Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, 631-8505, Japan; Magara, L., Department of Applied Biologi...
推荐引用方式 GB/T 7714	Ihara M.,Furutani S.,Shigetou S.,et al. Cofactor-enabled functional expression of fruit fly; honeybee; and bumblebee nicotinic receptors reveals picomolar neonicotinoid actions[J],2020,117(28).
APA	Ihara M..,Furutani S..,Shigetou S..,Shimada S..,Niki K..,...&Matsuda K..(2020).Cofactor-enabled functional expression of fruit fly; honeybee; and bumblebee nicotinic receptors reveals picomolar neonicotinoid actions.Proceedings of the National Academy of Sciences of the United States of America,117(28).
MLA	Ihara M.,et al."Cofactor-enabled functional expression of fruit fly; honeybee; and bumblebee nicotinic receptors reveals picomolar neonicotinoid actions".Proceedings of the National Academy of Sciences of the United States of America 117.28(2020).