气候变化领域集成服务门户(CCPortal): Spemann organizer transcriptome induction by early beta-catenin; Wnt; Nodal; and Siamois signals in Xenopus laevis

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DOI	10.1073/pnas.1700766114
	Spemann organizer transcriptome induction by early beta-catenin; Wnt; Nodal; and Siamois signals in Xenopus laevis
	Ding Y.; Ploper D.; Sosa E.A.; Colozza G.; Moriyama Y.; Benitez M.D.J.; Zhang K.; Merkurjev D.; De Robertis E.M.
发表日期	2017
ISSN	0027-8424
起始页码	E3081
结束页码	E3090
卷号	114 期号:15
英文摘要	The earliest event in Xenopus development is the dorsal accumulation of nuclear β-catenin under the influence of cytoplasmic determinants displaced by fertilization. In this study, a genome-wide approach was used to examine transcription of the 43,673 genes annotated in the Xenopus laevis genome under a variety of conditions that inhibit or promote formation of the Spemann organizer signaling center. Loss of function of β-catenin with antisense morpholinos reproducibly reduced the expression of 247 mRNAs at gastrula stage. Interestingly, only 123 β-catenin targets were enriched on the dorsal side and defined an early dorsal β-catenin gene signature. These genes included several previously unrecognized Spemann organizer components. Surprisingly, only 3 of these 123 genes overlapped with the late Wnt signature recently defined by two other groups using inhibition by Dkk1 mRNA or Wnt8 morpholinos, which indicates that the effects of β-catenin/ Wnt signaling in early development are exquisitely regulated by stage-dependent mechanisms. We analyzed transcriptome responses to a number of treatments in a total of 46 RNA-seq libraries. These treatments included, in addition to β-catenin depletion, regenerating dorsal and ventral half-embryos, lithium chloride treatment, and the overexpression of Wnt8, Siamois, and Cerberus mRNAs. Only some of the early dorsal β-catenin signature genes were activated at blastula whereas others required the induction of endomesoderm, as indicated by their inhibition by Cerberus overexpression. These comprehensive data provide a rich resource for analyzing how the dorsal and ventral regions of the embryo communicate with each other in a self-organizing vertebrate model embryo. © 2017, National Academy of Sciences. All rights reserved.
英文关键词	Cerberus; Embryonic induction; Embryonic patterning; Spemann organizer; Wnt signaling
语种	英语
scopus关键词	beta catenin; dickkopf 1 protein; lithium chloride; messenger RNA; morpholino oligonucleotide; RNA; transcriptome; Wnt8a protein; beta catenin; homeodomain protein; protein Nodal; siamois protein, Xenopus; transcriptome; Wnt protein; Xenopus protein; Article; blastula; embryo; gastrula; genome; morphogenesis; nonhuman; priority journal; signal transduction; Spemann organizer; transcription initiation; ventral region; Wnt signaling; Xenopus laevis; amino acid sequence; animal; gene expression regulation; genetics; growth, development and aging; metabolism; physiology; sequence homology; Spemann organizer; Xenopus laevis; Amino Acid Sequence; Animals; beta Catenin; Gene Expression Regulation, Developmental; Homeodomain Proteins; Nodal Protein; Organizers, Embryonic; Sequence Homology; Transcriptome; Wnt Proteins; Xenopus laevis; Xenopus Proteins
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160646
作者单位	Ding, Y., Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095-1662, United States, Department of Biological Chemistry, University of California, Los Angeles, CA 90095-1662, United States; Ploper, D., Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095-1662, United States, Department of Biological Chemistry, University of California, Los Angeles, CA 90095-1662, United States; Sosa, E.A., Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095-1662, United States, Department of Biological Chemistry, University of California, Los Angeles, CA 90095-1662, United States; Colozza, G., Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095-1662, United States, Department of Biological Chemistry, University of California, Los Angeles, CA 90095-1662, United States; Moriyama, Y., Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095-1662, United States, Department of Biolog...
推荐引用方式 GB/T 7714	Ding Y.,Ploper D.,Sosa E.A.,et al. Spemann organizer transcriptome induction by early beta-catenin; Wnt; Nodal; and Siamois signals in Xenopus laevis[J],2017,114(15).
APA	Ding Y..,Ploper D..,Sosa E.A..,Colozza G..,Moriyama Y..,...&De Robertis E.M..(2017).Spemann organizer transcriptome induction by early beta-catenin; Wnt; Nodal; and Siamois signals in Xenopus laevis.Proceedings of the National Academy of Sciences of the United States of America,114(15).
MLA	Ding Y.,et al."Spemann organizer transcriptome induction by early beta-catenin; Wnt; Nodal; and Siamois signals in Xenopus laevis".Proceedings of the National Academy of Sciences of the United States of America 114.15(2017).