气候变化领域集成服务门户(CCPortal): Arabidopsis ABCG34 contributes to defense against necrotrophic pathogens by mediating the secretion of camalexin

CCPortal

DOI	10.1073/pnas.1702259114
	Arabidopsis ABCG34 contributes to defense against necrotrophic pathogens by mediating the secretion of camalexin
	Khare D.; Choi H.; Huh S.U.; Bassin B.; Kim J.; Martinoia E.; Sohn K.H.; Paek K.-H.; Lee Y.; Chrispeels M.J.
发表日期	2017
ISSN	0027-8424
起始页码	E5712
结束页码	E5720
卷号	114 期号:28
英文摘要	Plant pathogens cause huge yield losses. Plant defense often depends on toxic secondary metabolites that inhibit pathogen growth. Because most secondary metabolites are also toxic to the plant, specific transporters are needed to deliver them to the pathogens. To identify the transporters that function in plant defense, we screened Arabidopsis thaliana mutants of full-size ABCG transporters for hypersensitivity to sclareol, an antifungal compound. We found that atabcg34 mutants were hypersensitive to sclareol and to the necrotrophic fungi Alternaria brassicicola and Botrytis cinerea. AtABCG34 expression was induced by A. brassicicola inoculation as well as by methyl-jasmonate, a defense-related phytohormone, and AtABCG34 was polarly localized at the external face of the plasma membrane of epidermal cells of leaves and roots. atabcg34 mutants secreted less camalexin, a major phytoalexin in A. thaliana, whereas plants overexpressing AtABCG34 secreted more camalexin to the leaf surface and were more resistant to the pathogen. When treated with exogenous camalexin, atabcg34 mutants exhibited hypersensitivity, whereas BY2 cells expressing AtABCG34 exhibited improved resistance. Analyses of natural Arabidopsis accessions revealed that AtABCG34 contributes to the disease resistance in naturally occurring genetic variants, albeit to a small extent. Together, our data suggest that AtABCG34 mediates camalexin secretion to the leaf surface and thereby prevents A. brassicicola infection.
英文关键词	A. brassicicola; ABC transporters; ATABCG34; B. cinerea; Camalexin
语种	英语
scopus关键词	abcg transporter 34; Arabidopsis protein; camalexin; carrier proteins and binding proteins; jasmonic acid methyl ester; phytoalexin; phytohormone; unclassified drug; acetic acid derivative; Arabidopsis protein; camalexin; cyclopentane derivative; diterpene; green fluorescent protein; indole derivative; oxylipin; sclareol; thiazole derivative; Alternaria brassicicola; Arabidopsis thaliana; Article; Botrytis cinerea; cell membrane; genetic variability; nonhuman; plant defense; plant disease; plant epidermis cell; plant leaf; plant root; priority journal; protein expression; protein secretion; Alternaria; Arabidopsis; Botrytis; gene expression profiling; gene expression regulation; metabolism; microbiology; mutation; pathogenicity; phenotype; phylogeny; plant disease; signal transduction; transport at the cellular level; Acetates; Alternaria; Arabidopsis; Arabidopsis Proteins; Biological Transport; Botrytis; Cyclopentanes; Diterpenes; Gene Expression Profiling; Gene Expression Regulation, Plant; Green Fluorescent Proteins; Indoles; Mutation; Oxylipins; Phenotype; Phylogeny; Plant Diseases; Plant Leaves; Signal Transduction; Thiazoles
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160617
作者单位	Khare, D., Department of Life Sciences, Pohang University of Science and Technology, Pohang, 37673, South Korea; Choi, H., Department of Life Sciences, Pohang University of Science and Technology, Pohang, 37673, South Korea; Huh, S.U., Department of Life Sciences, Korea University, Seoul, 02841, South Korea, Sainsbury Laboratory, Norwich, NR4 7UH, United Kingdom; Bassin, B., Department of Plant and Microbial Biology, University of Zurich, Zurich, 8008, Switzerland; Kim, J., Center for Plant Aging Research, Institute for Basic Science, Daegu, 42988, South Korea; Martinoia, E., Department of Plant and Microbial Biology, University of Zurich, Zurich, 8008, Switzerland; Sohn, K.H., Department of Life Sciences, Pohang University of Science and Technology, Pohang, 37673, South Korea, School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, 37673, South Korea; Paek, K.-H., Department of Life Sciences, Korea University, Seoul, 02841, South Korea; Lee, Y., Dep...
推荐引用方式 GB/T 7714	Khare D.,Choi H.,Huh S.U.,et al. Arabidopsis ABCG34 contributes to defense against necrotrophic pathogens by mediating the secretion of camalexin[J],2017,114(28).
APA	Khare D..,Choi H..,Huh S.U..,Bassin B..,Kim J..,...&Chrispeels M.J..(2017).Arabidopsis ABCG34 contributes to defense against necrotrophic pathogens by mediating the secretion of camalexin.Proceedings of the National Academy of Sciences of the United States of America,114(28).
MLA	Khare D.,et al."Arabidopsis ABCG34 contributes to defense against necrotrophic pathogens by mediating the secretion of camalexin".Proceedings of the National Academy of Sciences of the United States of America 114.28(2017).