气候变化领域集成服务门户(CCPortal): Virulence protein VirD5 of Agrobacterium tumefaciens binds to kinetochores in host cells via an interaction with Spt4

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DOI	10.1073/pnas.1706166114
	Virulence protein VirD5 of Agrobacterium tumefaciens binds to kinetochores in host cells via an interaction with Spt4
	Zhang X.; Van Heusden G.P.H.; Hooykaas P.J.J.
发表日期	2017
ISSN	0027-8424
起始页码	10238
结束页码	10243
卷号	114 期号:38
英文摘要	The bacterium Agrobacterium tumefaciens causes crown gall tumor formation in plants. During infection the bacteria translocate an oncogenic piece of DNA (transferred DNA, T-DNA) into plant cells at the infection site. A number of virulence proteins are cotransported into host cells concomitantly with the T-DNA to effectuate transformation. Using yeast as a model host, we find that one of these proteins, VirD5, localizes to the centromeres/kinetochores in the nucleus of the host cells by its interaction with the conserved protein Spt4. VirD5 promotes chromosomal instability as seen by the high-frequency loss of a minichromosome in yeast. By using both yeast and plant cells with a chromosome that was specifically marked by a lacO repeat, chromosome segregation errors and the appearance of aneuploid cells due to the presence of VirD5 could be visualized in vivo. Thus, VirD5 is a prokaryotic virulence protein that interferes with mitosis. © 2017, National Academy of Sciences. All rights reserved.
英文关键词	Agrobacterium; Kinetochore; Mitosis; Spt4; Virulence
语种	英语
scopus关键词	bacterial protein; spt4 protein; unclassified drug; vird5 protein; Arabidopsis protein; bacterial protein; SPT4 protein, Arabidopsis; transcription elongation factor; Agrobacterium tumefaciens; Article; bacterial virulence; cell nucleus; chromosomal instability; chromosome segregation; growth inhibition; host cell; kinetochore; minichromosome; mitosis; nonhuman; priority journal; protein binding; protein protein interaction; Agrobacterium tumefaciens; amino acid sequence; Arabidopsis; kinetochore; metabolism; pathogenicity; physiology; Agrobacterium tumefaciens; Amino Acid Sequence; Arabidopsis; Arabidopsis Proteins; Bacterial Proteins; Chromosome Segregation; Kinetochores; Transcriptional Elongation Factors
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160592
作者单位	Zhang, X., Department of Molecular and Developmental Genetics, Institute of Biology, Leiden University, Leiden, 2333BE, Netherlands; Van Heusden, G.P.H., Department of Molecular and Developmental Genetics, Institute of Biology, Leiden University, Leiden, 2333BE, Netherlands; Hooykaas, P.J.J., Department of Molecular and Developmental Genetics, Institute of Biology, Leiden University, Leiden, 2333BE, Netherlands
推荐引用方式 GB/T 7714	Zhang X.,Van Heusden G.P.H.,Hooykaas P.J.J.. Virulence protein VirD5 of Agrobacterium tumefaciens binds to kinetochores in host cells via an interaction with Spt4[J],2017,114(38).
APA	Zhang X.,Van Heusden G.P.H.,&Hooykaas P.J.J..(2017).Virulence protein VirD5 of Agrobacterium tumefaciens binds to kinetochores in host cells via an interaction with Spt4.Proceedings of the National Academy of Sciences of the United States of America,114(38).
MLA	Zhang X.,et al."Virulence protein VirD5 of Agrobacterium tumefaciens binds to kinetochores in host cells via an interaction with Spt4".Proceedings of the National Academy of Sciences of the United States of America 114.38(2017).