气候变化领域集成服务门户(CCPortal): Molecular architecture of the sheathed polar flagellum in Vibrio alginolyticus

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DOI	10.1073/pnas.1712489114
	Molecular architecture of the sheathed polar flagellum in Vibrio alginolyticus
	Zhu S.; Nishikino T.; Hu B.; Kojima S.; Homma M.; Liu J.
发表日期	2017
ISSN	0027-8424
起始页码	10966
结束页码	10971
卷号	114 期号:41
英文摘要	Vibrio species are Gram-negative rod-shaped bacteria that are ubiquitous and often highly motile in aqueous environments. Vibrio swimming motility is driven by a polar flagellum covered with a membranous sheath, but this sheathed flagellum is not well understood at the molecular level because of limited structural information. Here, we use Vibrio alginolyticus as a model system to study the sheathed flagellum in intact cells by combining cryoelectron tomography (cryo-ET) and subtomogram analysis with a genetic approach. We reveal striking differences between sheathed and unsheathed flagella in V. alginolyticus cells, including a novel ring-like structure at the bottom of the hook that is associated with major remodeling of the outer membrane and sheath formation. Using mutants defective in flagellar motor components, we defined a Vibrio-specific feature (also known as the T ring) as a distinctive periplasmic structure with 13-fold symmetry. The unique architecture of the T ring provides a static platform to recruit the PomA/B complexes, which are required to generate higher torques for rotation of the sheathed flagellum and fast motility of Vibrio cells. Furthermore, the Vibrio flagellar motor exhibits an intrinsic length variation between the inner and the outer membrane bound complexes, suggesting the outer membrane bound complex can shift slightly along the axial rod during flagellar rotation. Together, our detailed analyses of the polar flagella in intact cells provide insights into unique aspects of the sheathed flagellum and the distinct motility of Vibrio species. © 2017, National Academy of Sciences. All rights reserved.
英文关键词	Electron tomography; Flagellum; Nanomachine; Vibrio
语种	英语
scopus关键词	amino terminal sequence; Article; bacterial cell; carboxy terminal sequence; cell membrane; cell motility; conformational transition; controlled study; electron tomography; Escherichia coli; flagellum; high throughput screening; inner membrane; molecular dynamics; nonhuman; outer membrane; priority journal; structure analysis; Vibrio alginolyticus; cytology; flagellum; genetics; metabolism; procedures; ultrastructure; Vibrio alginolyticus; bacterial protein; sodium; Bacterial Proteins; Electron Microscope Tomography; Flagella; Sodium; Vibrio alginolyticus
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160589
作者单位	Zhu, S., Department of Pathology and Laboratory Medicine, McGovern Medical School, University of Texas Health Science Center at Houston, Houston, TX 77030, United States, Department of Microbial Pathogenesis, Microbial Sciences Institute, Yale School of Medicine, New Haven, CT 06536, United States; Nishikino, T., Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya, 464-8602, Japan; Hu, B., Department of Pathology and Laboratory Medicine, McGovern Medical School, University of Texas Health Science Center at Houston, Houston, TX 77030, United States; Kojima, S., Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya, 464-8602, Japan; Homma, M., Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya, 464-8602, Japan; Liu, J., Department of Pathology and Laboratory Medicine, McGovern Medical School, University of Texas Health Science Center at Houston, Houston, TX 77030, Unit...
推荐引用方式 GB/T 7714	Zhu S.,Nishikino T.,Hu B.,et al. Molecular architecture of the sheathed polar flagellum in Vibrio alginolyticus[J],2017,114(41).
APA	Zhu S.,Nishikino T.,Hu B.,Kojima S.,Homma M.,&Liu J..(2017).Molecular architecture of the sheathed polar flagellum in Vibrio alginolyticus.Proceedings of the National Academy of Sciences of the United States of America,114(41).
MLA	Zhu S.,et al."Molecular architecture of the sheathed polar flagellum in Vibrio alginolyticus".Proceedings of the National Academy of Sciences of the United States of America 114.41(2017).