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DOI	10.1073/pnas.1708054114
	Speed of the bacterial flagellar motor near zero load depends on the number of stator units
	Nord A.L.; Sowa Y.; Steel B.C.; Lo C.-J.; Berry R.M.
发表日期	2017
ISSN	0027-8424
起始页码	11603
结束页码	11608
卷号	114 期号:44
英文摘要	The bacterial flagellar motor (BFM) rotates hundreds of times per second to propel bacteria driven by an electrochemical ion gradient. The motor consists of a rotor 50 nm in diameter surrounded by up to 11 ion-conducting stator units, which exchange between motors and a membrane-bound pool. Measurements of the torque–speed relationship guide the development of models of the motor mechanism. In contrast to previous reports that speed near zero torque is independent of the number of stator units, we observe multiple speeds that we attribute to different numbers of units near zero torque in both Na+- and H+driven motors. We measure the full torque–speed relationship of one and two H+ units in Escherichia coli by selecting the number of H+ units and controlling the number of Na+ units in hybrid motors. These experiments confirm that speed near zero torque in H+-driven motors increases with the stator number. We also measured 75 torque–speed curves for Na+-driven chimeric motors at different ion-motive force and stator number. Torque and speed were proportional to ion-motive force and number of stator units at all loads, allowing all 77 measured torque–speed curves to be collapsed onto a single curve by simple rescaling. © 2017, National Academy of Sciences. All rights reserved.
英文关键词	Bacterial flagellar motor; Escherichia coli; Hybrid fuel motor; Molecular motor; Motility
语种	英语
scopus关键词	bacterial flagellar motor; gold nanoparticle; hydrogen; molecular motor; polystyrene; sodium ion; unclassified drug; molecular motor; sodium; Article; bacterial flagellum; electric potential; Escherichia coli; force; histogram; nonhuman; priority journal; speed near zero torque; stator unit; torque; velocity; biomechanics; flagellum; physiology; Biomechanical Phenomena; Escherichia coli; Flagella; Molecular Motor Proteins; Sodium; Torque
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160581
作者单位	Nord, A.L., Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, OX1 3PU, United Kingdom, Single Molecule Biophysics Department, Centre de Biochimie Stucturale, CNRS, UMR 5048, UM INSERM U 1054, Montpellier, 34090, France; Sowa, Y., Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, OX1 3PU, United Kingdom, Department of Frontier Bioscience, Hosei University, Tokyo, 184-8584, Japan; Steel, B.C., Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, OX1 3PU, United Kingdom; Lo, C.-J., Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, OX1 3PU, United Kingdom, Department of Physics, National Central University, Jhongli, 32001, Taiwan, Graduate Institute of Biophysics, National Central University, Jhongli, 32001, Taiwan; Berry, R.M., Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, OX1 3PU, United Kingdom
推荐引用方式 GB/T 7714	Nord A.L.,Sowa Y.,Steel B.C.,et al. Speed of the bacterial flagellar motor near zero load depends on the number of stator units[J],2017,114(44).
APA	Nord A.L.,Sowa Y.,Steel B.C.,Lo C.-J.,&Berry R.M..(2017).Speed of the bacterial flagellar motor near zero load depends on the number of stator units.Proceedings of the National Academy of Sciences of the United States of America,114(44).
MLA	Nord A.L.,et al."Speed of the bacterial flagellar motor near zero load depends on the number of stator units".Proceedings of the National Academy of Sciences of the United States of America 114.44(2017).