气候变化领域集成服务门户(CCPortal): CSI1; PATROL1; and exocyst complex cooperate in delivery of cellulose synthase complexes to the plasma membrane

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DOI	10.1073/pnas.1800182115
	CSI1; PATROL1; and exocyst complex cooperate in delivery of cellulose synthase complexes to the plasma membrane
	Zhu X.; Li S.; Pan S.; Xin X.; Gu Y.
发表日期	2018
ISSN	0027-8424
起始页码	E3578
结束页码	E3587
卷号	115 期号:15
英文摘要	Cellulose synthesis occurs exclusively at the plasma membrane by cellulose synthase complexes (CSCs). Therefore, delivery of CSCs to discrete sites at the plasma membrane is critical for cellulose synthesis. Despite their significance, the delivery of CSCs is poorly understood. Here we used proteomics approaches, functional genetics, and live cell imaging to show that the de novo secretion of CSCs is mediated by cooperation among cellulose synthase interactive 1 (CSI1), the plant-specific protein PATROL1, and exocyst complex in Arabidopsis thaliana. We propose that CSI1 plays a role in marking the docking site, which allows CSCs-containing vesicles access to the plasma membrane through its interaction with microtubules. PATROL1 assists in exocytosis by its interaction with multiple components, including CSI1, CSCs, and exocyst subunits. Both PATROL1 and the exocyst complex determine the rate of delivery of CSCs to the plasma membrane. By monitoring the exocyst complex, PATROL1, CSI1, and CSCs dynamics in real time, we present a timeline of events for exocytosis of CSCs. Our findings provide unique insights into the evolution of exocytosis in eukaryotes. © 2018 National Academy of Sciences. All Rights Reserved.
英文关键词	Cellulose synthase complex; Exocyst complex; Exocytosis; Microtubule; Plasma membrane
语种	英语
scopus关键词	Arabidopsis protein; cellulose synthase complex; cellulose synthase interactive 1; exocyst; PATROL1 protein; unclassified drug; Arabidopsis protein; carrier protein; cellulose; cellulose synthase; CSI1 protein, Arabidopsis; glucosyltransferase; PATROL1 protein, Arabidopsis; vesicular transport protein; Arabidopsis thaliana; Article; cell membrane; cell vacuole; controlled study; exocytosis; live cell imaging; microtubule; nonhuman; plant cell; plant genetics; priority journal; protein function; protein protein interaction; protein secretion; protein transport; proteomics; Arabidopsis; biosynthesis; cell membrane; cytoplasm; metabolism; protein transport; Arabidopsis; Arabidopsis Proteins; Carrier Proteins; Cell Membrane; Cellulose; Cytoplasm; Glucosyltransferases; Microtubules; Protein Transport; Vesicular Transport Proteins
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160555
作者单位	Zhu, X., Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, United States; Li, S., Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, United States; Pan, S., W. M. Keck Proteomics Laboratory, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, United States, Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, United States; Xin, X., Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, United States; Gu, Y., Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, United States
推荐引用方式 GB/T 7714	Zhu X.,Li S.,Pan S.,et al. CSI1; PATROL1; and exocyst complex cooperate in delivery of cellulose synthase complexes to the plasma membrane[J],2018,115(15).
APA	Zhu X.,Li S.,Pan S.,Xin X.,&Gu Y..(2018).CSI1; PATROL1; and exocyst complex cooperate in delivery of cellulose synthase complexes to the plasma membrane.Proceedings of the National Academy of Sciences of the United States of America,115(15).
MLA	Zhu X.,et al."CSI1; PATROL1; and exocyst complex cooperate in delivery of cellulose synthase complexes to the plasma membrane".Proceedings of the National Academy of Sciences of the United States of America 115.15(2018).