气候变化领域集成服务门户(CCPortal): Cooption of heat shock regulatory system for anhydrobiosis in the sleeping chironomid Polypedilum vanderplanki

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DOI	10.1073/pnas.1719493115
	Cooption of heat shock regulatory system for anhydrobiosis in the sleeping chironomid Polypedilum vanderplanki
	Mazin P.V.; Shagimardanova E.; Kozlova O.; Cherkasov A.; Sutormin R.; Stepanova V.V.; Stupnikov A.; Logacheva M.; Penin A.; Sogame Y.; Cornette R.; Tokumoto S.; Miyata Y.; Kikawada T.; Gelfand M.S.; Gusev O.
发表日期	2018
ISSN	0027-8424
起始页码	E2477
结束页码	E2486
卷号	115 期号:10
英文摘要	Polypedilum vanderplanki is a striking and unique example of an insect that can survive almost complete desiccation. Its genome and a set of dehydration–rehydration transcriptomes, together with the genome of Polypedilum nubifer (a congeneric desiccation-sensitive midge), were recently released. Here, using published and newly generated datasets reflecting detailed transcriptome changes during anhydrobiosis, as well as a developmental series, we show that the TCTAGAA DNA motif, which closely resembles the binding motif of the Drosophila melanogaster heat shock transcription activator (Hsf), is significantly enriched in the promoter regions of desiccation-induced genes in P. vanderplanki, such as genes encoding late embryogenesis abundant (LEA) proteins, thioredoxins, or trehalose metabolism-related genes, but not in P. nubifer. Unlike P. nubifer, P. vanderplanki has double TCTAGAA sites upstream of the Hsf gene itself, which is probably responsible for the stronger activation of Hsf in P. vanderplanki during desiccation compared with P. nubifer. To confirm the role of Hsf in desiccation-induced gene activation, we used the Pv11 cell line, derived from P. vanderplanki embryo. After preincubation with trehalose, Pv11 cells can enter anhydrobiosis and survive desiccation. We showed that Hsf knockdown suppresses trehalose-induced activation of multiple predicted Hsf targets (including P. vanderplanki-specific LEA protein genes) and reduces the desiccation survival rate of Pv11 cells fivefold. Thus, cooption of the heat shock regulatory system has been an important evolutionary mechanism for adaptation to desiccation in P. vanderplanki. © 2018 National Academy of Sciences. All Rights Reserved.
英文关键词	Anhydrobiosis; Desiccation tolerance; Heat shock; Polypedilum vanderplanki; RNA-seq
语种	英语
scopus关键词	cell protein; heat shock transcription factor; late embryogenesis abundant protein; thioredoxin; transcriptome; trehalose; unclassified drug; heat shock transcription factor; insect protein; anhydrobiosis; Article; carbohydrate metabolism; cell line; Chironomidae; desiccation; DNA structure; Drosophila melanogaster; gene activation; gene function; gene induction; gene knockdown; insect development; nonhuman; Polypedilum nubifer; Polypedilum vanderplanki; priority journal; promoter region; Pv11 cell line; species comparison; survival rate; animal; Chironomidae; dehydration; evolution; female; genetics; heat shock response; male; metabolism; physiological stress; physiology; Animals; Biological Evolution; Chironomidae; Dehydration; Female; Heat Shock Transcription Factors; Heat-Shock Response; Insect Proteins; Male; Stress, Physiological
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160539
作者单位	Mazin, P.V., Center for Data-Intensive Biomedicine and Biotechnology, Skolkovo Institute of Science and Technology, Moscow, 143028, Russian Federation, Institute for Information Transmission Problems (Kharkevich Institute) RAS, Moscow, 127051, Russian Federation, Faculty of Computer Science, Higher School of Economics, Moscow, 119991, Russian Federation; Shagimardanova, E., Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, 420012, Russian Federation; Kozlova, O., Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, 420012, Russian Federation; Cherkasov, A., Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, 420012, Russian Federation; Sutormin, R., Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94710, United States; Stepanova, V.V., Center for Data-Intensive Biomedicine and Biotechnology, Skolkovo Institute of Science and Technology, Moscow, 143028, Russian Federation, Institute for Inform...
推荐引用方式 GB/T 7714	Mazin P.V.,Shagimardanova E.,Kozlova O.,et al. Cooption of heat shock regulatory system for anhydrobiosis in the sleeping chironomid Polypedilum vanderplanki[J],2018,115(10).
APA	Mazin P.V..,Shagimardanova E..,Kozlova O..,Cherkasov A..,Sutormin R..,...&Gusev O..(2018).Cooption of heat shock regulatory system for anhydrobiosis in the sleeping chironomid Polypedilum vanderplanki.Proceedings of the National Academy of Sciences of the United States of America,115(10).
MLA	Mazin P.V.,et al."Cooption of heat shock regulatory system for anhydrobiosis in the sleeping chironomid Polypedilum vanderplanki".Proceedings of the National Academy of Sciences of the United States of America 115.10(2018).