气候变化领域集成服务门户(CCPortal): MYB72-dependent coumarin exudation shapes root microbiome assembly to promote plant health

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DOI	10.1073/pnas.1722335115
	MYB72-dependent coumarin exudation shapes root microbiome assembly to promote plant health
	Stringlis I.A.; Yu K.; Feussner K.; De Jonge R.; Van Bentum S.; Van Verk M.C.; Berendsen R.L.; Bakker P.A.H.M.; Feussner I.; Pieterse C.M.J.
发表日期	2018
ISSN	0027-8424
起始页码	E5213
结束页码	E5222
卷号	115 期号:22
英文摘要	Plant roots nurture a tremendous diversity of microbes via exudation of photosynthetically fixed carbon sources. In turn, probiotic members of the root microbiome promote plant growth and protect the host plant against pathogens and pests. In the Arabidopsis thaliana–Pseudomonas simiae WCS417 model system the root-specific transcription factor MYB72 and the MYB72-controlled β-glucosidase BGLU42 emerged as important regulators of beneficial rhizobacteria-induced systemic resistance (ISR) and iron-uptake responses. MYB72 regulates the biosynthesis of iron-mobilizing fluorescent phenolic compounds, after which BGLU42 activity is required for their excretion into the rhizosphere. Metabolite fingerprinting revealed the antimicrobial coumarin scopoletin as a dominant metabolite that is produced in the roots and excreted into the rhizosphere in a MYB72- and BGLU42-dependent manner. Shotgun-metagenome sequencing of root-associated microbiota of Col-0, myb72, and the scopoletin biosynthesis mutant f6′h1 showed that scopoletin selectively impacts the assembly of the microbial community in the rhizosphere. We show that scopoletin selectively inhibits the soil-borne fungal pathogens Fusarium oxysporum and Verticillium dahliae, while the growth-promoting and ISR-inducing rhizobacteria P. simiae WCS417 and Pseudomonas capeferrum WCS358 are highly tolerant of the antimicrobial effect of scopoletin. Collectively, our results demonstrate a role for coumarins in microbiome assembly and point to a scenario in which plants and probiotic rhizobacteria join forces to trigger MYB72/BGLU42-dependent scopolin production and scopoletin excretion, resulting in improved niche establishment for the microbial partner and growth and immunity benefits for the host plant. © 2018 National Academy of Sciences. All rights reserved.
英文关键词	Coumarin; Induced systemic resistance; Iron-deficiency response; Microbiome assembly; Root metabolome
语种	英语
scopus关键词	beta glucosidase; coumarin; glucoside; protein BGLU42; protein MYB72; scopoletin; scopolin; transcription factor; unclassified drug; Arabidopsis protein; iron; Myb72 protein, Arabidopsis; scopoletin; transcription factor; Arabidopsis thaliana; Article; bacterial microbiome; controlled study; enzyme activity; Fusarium oxysporum; gene sequence; metagenome; microbial community; microbiome assembly; mycobiome; nonhuman; organismal interaction; plant health; plant immunity; plant physiology; plant root; priority journal; protein function; protein synthesis; Pseudomonas; Pseudomonas capeferrum; Pseudomonas simiae; rhizosphere; Verticillium dahliae; Arabidopsis; Fusarium; metabolism; metabolome; microbiology; microflora; physiology; Verticillium; Arabidopsis; Arabidopsis Proteins; Fusarium; Iron; Metabolome; Microbiota; Plant Roots; Pseudomonas; Rhizosphere; Scopoletin; Transcription Factors; Verticillium
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160508
作者单位	Stringlis, I.A., Plant–Microbe Interactions, Department of Biology, Science4Life, Utrecht University, Utrecht, 3508 TB, Netherlands; Yu, K., Plant–Microbe Interactions, Department of Biology, Science4Life, Utrecht University, Utrecht, 3508 TB, Netherlands; Feussner, K., Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, University of Göttingen, Göttingen, 37077, Germany; De Jonge, R., Plant–Microbe Interactions, Department of Biology, Science4Life, Utrecht University, Utrecht, 3508 TB, Netherlands, Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, Ghent, 9052, Belgium, Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, 9052, Belgium; Van Bentum, S., Plant–Microbe Interactions, Department of Biology, Science4Life, Utrecht University, Utrecht, 3508 TB, Netherlands; Van Verk, M.C., Plant–Microbe Interactions, Department of Biology, Science4Life, Utrecht University, Utrecht, 3508 TB, Netherlands; Berendsen, R.L., Plant–Micro...
推荐引用方式 GB/T 7714	Stringlis I.A.,Yu K.,Feussner K.,et al. MYB72-dependent coumarin exudation shapes root microbiome assembly to promote plant health[J],2018,115(22).
APA	Stringlis I.A..,Yu K..,Feussner K..,De Jonge R..,Van Bentum S..,...&Pieterse C.M.J..(2018).MYB72-dependent coumarin exudation shapes root microbiome assembly to promote plant health.Proceedings of the National Academy of Sciences of the United States of America,115(22).
MLA	Stringlis I.A.,et al."MYB72-dependent coumarin exudation shapes root microbiome assembly to promote plant health".Proceedings of the National Academy of Sciences of the United States of America 115.22(2018).