气候变化领域集成服务门户(CCPortal): Growth suppression of ice crystal basal face in the presence of a moderate ice-binding protein does not confer hyperactivity

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DOI	10.1073/pnas.1807461115
	Growth suppression of ice crystal basal face in the presence of a moderate ice-binding protein does not confer hyperactivity
	Bayer-Giraldi M.; Sazaki G.; Nagashima K.; Kipfstuhl S.; Vorontsov D.A.; Furukawa Y.
发表日期	2018
ISSN	0027-8424
起始页码	7479
结束页码	7484
卷号	115 期号:29
英文摘要	Ice-binding proteins (IBPs) affect ice crystal growth by attaching to crystal faces. We present the effects on the growth of an ice single crystal caused by an ice-binding protein from the sea ice microalga Fragilariopsis cylindrus (fcIBP) that is characterized by the widespread domain of unknown function 3494 (DUF3494) and known to cause a moderate freezing point depression (below 1 °C). By the application of interferometry, bright-field microscopy, and fluorescence microscopy, we observed that the fcIBP attaches to the basal faces of ice crystals, thereby inhibiting their growth in the c direction and resulting in an increase in the effective supercooling with increasing fcIBP concentration. In addition, we observed that the fcIBP attaches to prism faces and inhibits their growth. In the event that the effective supercooling is small and crystals are faceted, this process causes an emergence of prism faces and suppresses crystal growth in the a direction. When the effective supercooling is large and ice crystals have developed into a dendritic shape, the suppression of prism face growth results in thinner dendrite branches, and growth in the a direction is accelerated due to enhanced latent heat dissipation. Our observations clearly indicate that the fcIBP occupies a separate position in the classification of IBPs due to the fact that it suppresses the growth of basal faces, despite its moderate freezing point depression. © 2018 National Academy of Sciences. All Rights Reserved.
英文关键词	DUF3494; Fragilariopsis cylindrus; Growth rates; Ice crystallization; Ice-binding protein
语种	英语
scopus关键词	binding protein; ice binding protein; unclassified drug; algal protein; ice; Article; bright field microscopy; cell growth; confocal laser scanning microscopy; controlled study; crystallization; fluorescence microscopy; Fragilariopsis cylindrus; freezing; growth inhibition; interferometry; melting point; microalga; nonhuman; priority journal; protein expression; protein function; protein localization; thickness; chemistry; diatom; freezing; metabolism; Algal Proteins; Diatoms; Freezing; Ice; Microalgae
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160490
作者单位	Bayer-Giraldi, M., Helmholtz Centre for Polar and Marine Research, Department of Glaciology (Geosciences), Alfred Wegener Institute, Bremerhaven, 27568, Germany, Institute of Low Temperature Science, Laboratory for Phase Transition Dynamics of Ice, Hokkaido University, Sapporo, 060-0819, Japan; Sazaki, G., Institute of Low Temperature Science, Laboratory for Phase Transition Dynamics of Ice, Hokkaido University, Sapporo, 060-0819, Japan; Nagashima, K., Institute of Low Temperature Science, Laboratory for Phase Transition Dynamics of Ice, Hokkaido University, Sapporo, 060-0819, Japan; Kipfstuhl, S., Helmholtz Centre for Polar and Marine Research, Department of Glaciology (Geosciences), Alfred Wegener Institute, Bremerhaven, 27568, Germany; Vorontsov, D.A., Institute of Low Temperature Science, Laboratory for Phase Transition Dynamics of Ice, Hokkaido University, Sapporo, 060-0819, Japan, Faculty of Physics, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, 603950, Russian Federation; Furuka...
推荐引用方式 GB/T 7714	Bayer-Giraldi M.,Sazaki G.,Nagashima K.,et al. Growth suppression of ice crystal basal face in the presence of a moderate ice-binding protein does not confer hyperactivity[J],2018,115(29).
APA	Bayer-Giraldi M.,Sazaki G.,Nagashima K.,Kipfstuhl S.,Vorontsov D.A.,&Furukawa Y..(2018).Growth suppression of ice crystal basal face in the presence of a moderate ice-binding protein does not confer hyperactivity.Proceedings of the National Academy of Sciences of the United States of America,115(29).
MLA	Bayer-Giraldi M.,et al."Growth suppression of ice crystal basal face in the presence of a moderate ice-binding protein does not confer hyperactivity".Proceedings of the National Academy of Sciences of the United States of America 115.29(2018).