气候变化领域集成服务门户(CCPortal): FtsZ filaments have the opposite kinetic polarity of microtubules

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DOI	10.1073/pnas.1811919115
	FtsZ filaments have the opposite kinetic polarity of microtubules
	Du S.; Pichoff S.; Kruse K.; Lutkenhaus J.
发表日期	2018
ISSN	0027-8424
起始页码	10768
结束页码	10773
卷号	115 期号:42
英文摘要	FtsZ is the ancestral homolog of tubulin and assembles into the Z ring that organizes the division machinery to drive cell division in most bacteria. In contrast to tubulin that assembles into 13 stranded microtubules that undergo dynamic instability, FtsZ assembles into single-stranded filaments that treadmill to distribute the peptidoglycan synthetic machinery at the septum. Here, using longitudinal interface mutants of FtsZ, we demonstrate that the kinetic polarity of FtsZ filaments is opposite to that of microtubules. A conformational switch accompanying the assembly of FtsZ generates the kinetic polarity of FtsZ filaments, which explains the toxicity of interface mutants that function as a capper and reveals the mechanism of cooperative assembly. This approach can also be employed to determine the kinetic polarity of other filament-forming proteins. © 2018 National Academy of Sciences. All Rights Reserved.
英文关键词	Capper; FtsZ; Treadmilling; Tubulin; Z ring
语种	英语
scopus关键词	bacterial protein; cytoskeleton protein; FtsZ protein, Bacteria; bacterium; chemistry; cytoskeleton; genetics; kinetics; metabolism; microtubule; mutation; polymerization; protein conformation; statistical model; Bacteria; Bacterial Proteins; Cytoskeletal Proteins; Cytoskeleton; Kinetics; Microtubules; Models, Statistical; Mutation; Polymerization; Protein Conformation
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160454
作者单位	Du, S., Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, United States; Pichoff, S., Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, United States; Kruse, K., Department of Biochemistry, University of Geneva, Geneva, 1211, Switzerland, Department of Theoretical Physics, University of Geneva, Geneva, 1211, Switzerland, National Center of Competence in Research Chemical Biology, University of Geneva, Geneva, 1211, Switzerland; Lutkenhaus, J., Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, United States
推荐引用方式 GB/T 7714	Du S.,Pichoff S.,Kruse K.,et al. FtsZ filaments have the opposite kinetic polarity of microtubules[J],2018,115(42).
APA	Du S.,Pichoff S.,Kruse K.,&Lutkenhaus J..(2018).FtsZ filaments have the opposite kinetic polarity of microtubules.Proceedings of the National Academy of Sciences of the United States of America,115(42).
MLA	Du S.,et al."FtsZ filaments have the opposite kinetic polarity of microtubules".Proceedings of the National Academy of Sciences of the United States of America 115.42(2018).