气候变化领域集成服务门户(CCPortal): Unexpected host dependency of Antarctic Nanohaloarchaeota

CCPortal

DOI	10.1073/pnas.1905179116
	Unexpected host dependency of Antarctic Nanohaloarchaeota
	Hamm J.N.; Erdmann S.; Eloe-Fadrosh E.A.; Angeloni A.; Zhong L.; Brownlee C.; Williams T.J.; Barton K.; Carswell S.; Smith M.A.; Brazendale S.; Hancock A.M.; Allen M.A.; Raftery M.J.; Cavicchioli R.
发表日期	2019
ISSN	0027-8424
起始页码	14661
结束页码	14670
卷号	116 期号:29
英文摘要	In hypersaline environments, Nanohaloarchaeota (Diapherotrites, Parvarchaeota, Aenigmarchaeota, Nanoarchaeota, Nanohaloarchaeota [DPANN] superphylum) are thought to be free-living microorganisms. We report cultivation of 2 strains of Antarctic Nanohaloarchaeota and show that they require the haloarchaeon Halorubrum lacusprofundi for growth. By performing growth using enrichments and fluorescence-activated cell sorting, we demonstrated successful cultivation of Candidatus Nanohaloarchaeum antarcticus, purification of Ca. Nha. antarcticus away from other species, and growth and verification of Ca. Nha. antarcticus with Hrr. lacusprofundi; these findings are analogous to those required for fulfilling Koch’s postulates. We use fluorescent in situ hybridization and transmission electron microscopy to assess cell structures and interactions; metagenomics to characterize enrichment taxa, generate metagenome assembled genomes, and interrogate Antarctic communities; and proteomics to assess metabolic pathways and speculate about the roles of certain proteins. Metagenome analysis indicates the presence of a single species, which is endemic to Antarctic hypersaline systems that support the growth of haloarchaea. The presence of unusually large proteins predicted to function in attachment and invasion of hosts plus the absence of key biosynthetic pathways (e.g., lipids) in metagenome assembled genomes of globally distributed Nanohaloarchaeota indicate that all members of the lineage have evolved as symbionts. Our work expands the range of archaeal symbiotic lifestyles and provides a genetically tractable model system for advancing understanding of the factors controlling microbial symbiotic relationships. © 2019 National Academy of Sciences. All rights reserved.
英文关键词	Archaea; DPANN; Symbiont
语种	英语
scopus关键词	Antarctic Nanohaloarchaeota; Article; bacterial genome; bacterial growth; carboxy terminal sequence; cell adhesion; controlled study; fluorescence activated cell sorting; fluorescence in situ hybridization; genetic variation; genome analysis; Halorubrum lacusprofundi; metagenome; metagenomics; Nanoarchaeota; nonhuman; priority journal; proteomics; species invasion; transmission electron microscopy
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160429
作者单位	Hamm, J.N., School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia; Erdmann, S., School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia, Department of Microbiology, Max Planck Institute for Marine Microbiology, Bremen, 28359, Germany; Eloe-Fadrosh, E.A., Department of Energy, Joint Genome Institute, Walnut Creek, CA 94598, United States; Angeloni, A., School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia; Zhong, L., Bioanalytical Mass Spectrometry Facility, University of New South Wales, Sydney, NSW 2052, Australia; Brownlee, C., Biological Resources Imaging Laboratory, University of New South Wales, Sydney, NSW 2052, Australia; Williams, T.J., School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia; Barton, K., Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Rese...
推荐引用方式 GB/T 7714	Hamm J.N.,Erdmann S.,Eloe-Fadrosh E.A.,et al. Unexpected host dependency of Antarctic Nanohaloarchaeota[J],2019,116(29).
APA	Hamm J.N..,Erdmann S..,Eloe-Fadrosh E.A..,Angeloni A..,Zhong L..,...&Cavicchioli R..(2019).Unexpected host dependency of Antarctic Nanohaloarchaeota.Proceedings of the National Academy of Sciences of the United States of America,116(29).
MLA	Hamm J.N.,et al."Unexpected host dependency of Antarctic Nanohaloarchaeota".Proceedings of the National Academy of Sciences of the United States of America 116.29(2019).