气候变化领域集成服务门户(CCPortal): Arabinogalactan protein–rare earth element complexes activate plant endocytosis

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DOI	10.1073/pnas.1902532116
	Arabinogalactan protein–rare earth element complexes activate plant endocytosis
	Wang L.; Cheng M.; Yang Q.; Li J.; Wang X.; Zhou Q.; Nagawa S.; Xia B.; Xu T.; Huang R.; He J.; Li C.; Fu Y.; Liu Y.; Bao J.; Wei H.; Li H.; Tan L.; Gu Z.; Xia A.; Huang X.; Yang Z.; Deng X.W.
发表日期	2019
ISSN	0027-8424
起始页码	14349
结束页码	14357
卷号	116 期号:28
英文摘要	Endocytosis is essential to all eukaryotes, but how cargoes are selected for internalization remains poorly characterized. Extracellular cargoes are thought to be selected by transmembrane receptors that bind intracellular adaptors proteins to initiate endocytosis. Here, we report a mechanism for clathrin-mediated endocytosis (CME) of extracellular lanthanum [La(III)] cargoes, which requires extracellular arabinogalactan proteins (AGPs) that are anchored on the outer face of the plasma membrane. AGPs were colocalized with La(III) on the cell surface and in La(III)induced endocytic vesicles in Arabidopsis leaf cells. Superresolution imaging showed that La(III) triggered AGP movement across the plasma membrane. AGPs were then colocalized and physically associated with the μ subunit of the intracellular adaptor protein 2 (AP2) complexes. The AGP-AP2 interaction was independent of CME, whereas AGP’s internalization required CME and AP2. Moreover, we show that AGP-dependent endocytosis in the presence of La(III) also occurred in human cells. These findings indicate that extracellular AGPs act as conserved CME cargo receptors, thus challenging the current paradigm about endocytosis of extracellular cargoes. © 2019 National Academy of Sciences. All rights reserved.
英文关键词	Arabinogalactan proteins; Endocytosis; Extracellular cargo; Lanthanum; Superresolution imaging
语种	英语
scopus关键词	adaptor protein; adaptor protein 2; arabinogalactan protein; glycoprotein; lanthanide; unclassified drug; Arabidopsis; Article; cell surface; cellular distribution; clathrin mediated endocytosis; complex formation; controlled study; endocytosis; endosome; HEK293 cell line; human; human cell; immunogold labeling; internalization; isotope labeling; nonhuman; plant leaf; priority journal; protein protein interaction
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160419
作者单位	Wang, L., National and Local Joint Engineering, Research Center of Biomedical Functional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China, State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China; Cheng, M., National and Local Joint Engineering, Research Center of Biomedical Functional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China; Yang, Q., National and Local Joint Engineering, Research Center of Biomedical Functional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China; Li, J., China State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agr...
推荐引用方式 GB/T 7714	Wang L.,Cheng M.,Yang Q.,等. Arabinogalactan protein–rare earth element complexes activate plant endocytosis[J],2019,116(28).
APA	Wang L..,Cheng M..,Yang Q..,Li J..,Wang X..,...&Deng X.W..(2019).Arabinogalactan protein–rare earth element complexes activate plant endocytosis.Proceedings of the National Academy of Sciences of the United States of America,116(28).
MLA	Wang L.,et al."Arabinogalactan protein–rare earth element complexes activate plant endocytosis".Proceedings of the National Academy of Sciences of the United States of America 116.28(2019).