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DOI	10.1073/pnas.1900401116
	Blastocyst activation engenders transcriptome reprogram affecting X-chromosome reactivation and inflammatory trigger of implantation
	He B.; Zhang H.; Wang J.; Liu M.; Sun Y.; Guo C.; Lu J.; Wang H.; Kong S.
发表日期	2019
ISSN	0027-8424
起始页码	16621
结束页码	16630
卷号	116 期号:33
英文摘要	Implantation of the blastocyst into the uterus is the gateway for further embryonic development in mammals. Programming of blastocyst to an implantation-competent state known as blastocyst activation is the determining factor for implantation into the receptive uterus. However, it remains largely unclear how the blastocyst is globally programmed for implantation. Employing a delayed implantation mouse model, we show here that the blastocyst undergoes extensive programming essential for implantation. By analyzing the transcriptional profile of blastocysts with different implantation competency, we reveal the dynamic change in the biosynthesis, metabolism, and proliferation during blastocyst reactivation from diapause. We also demonstrate that reactivation of the X chromosome, one of the most important events during periimplantation of female embryonic development, is not completed even in blastocysts under conditions of dormancy, despite long term suspension in the uterus. Moreover, the mural trophectoderm (TE), but not the polar TE, differentiates to be more invasive through the weakened cell-cell tight junctions and extracellular matrices (ECMs). By analyzing the differentially expressed profile of secretory proteins, we further demonstrate that the blastocyst functions as a proinflammatory body to secrete proinflammatory signals, such as TNFα and S100A9, thereby triggering embryouterine attachment reaction during implantation. Collectively, our data systematically and comprehensively disclose the programming of blastocyst reactivation from diapause for implantation and uncover previously undefined roles of blastocyst during implantation. © 2019 National Academy of Sciences. All rights reserved.
英文关键词	Blastocyst diapause and reactivation; Embryo implantation; Proinflammatory body; Transcriptome; X-chromosome reactivation
语种	英语
scopus关键词	calgranulin B; transcriptome; tumor necrosis factor; calgranulin B; transcriptome; tumor necrosis factor; animal cell; animal experiment; Article; biosynthesis; blastocyst; cell activation; cell differentiation; cell proliferation; cell proliferation assay; controlled study; diapause; dormancy; embryo development; endometrium; extracellular matrix; female; genetic transcription; metabolism; mouse; nonhuman; nuclear reprogramming; priority journal; protein expression; real time polymerase chain reaction; RNA sequence; signal transduction; tight junction; transcriptomics; upregulation; X chromosome linkage; animal; blastocyst; cytology; ectoderm; gene expression regulation; genetics; inflammation; nidation; pathology; time factor; ultrastructure; X chromosome; Animals; Blastocyst; Calgranulin B; Cell Proliferation; Ectoderm; Embryo Implantation; Endometrium; Female; Gene Expression Regulation, Developmental; Inflammation; Mice; Time Factors; Transcriptome; Tumor Necrosis Factor-alpha; Up-Regulation; X Chromosome
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160410
作者单位	He, B., Reproductive Medical Center, First Affiliated Hospital of Xiamen University, Xiamen, Fujian, 361003, China, Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, Fujian, 361102, China, State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China; Zhang, H., State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China, Basic Forestry and Proteomics Research Center, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China; Wang, J., Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, Fujian, 361102, China; Liu, M., Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, Fujian, 361102, China; Sun, Y., Reproductive Medical Center, First Affiliate...
推荐引用方式 GB/T 7714	He B.,Zhang H.,Wang J.,et al. Blastocyst activation engenders transcriptome reprogram affecting X-chromosome reactivation and inflammatory trigger of implantation[J],2019,116(33).
APA	He B..,Zhang H..,Wang J..,Liu M..,Sun Y..,...&Kong S..(2019).Blastocyst activation engenders transcriptome reprogram affecting X-chromosome reactivation and inflammatory trigger of implantation.Proceedings of the National Academy of Sciences of the United States of America,116(33).
MLA	He B.,et al."Blastocyst activation engenders transcriptome reprogram affecting X-chromosome reactivation and inflammatory trigger of implantation".Proceedings of the National Academy of Sciences of the United States of America 116.33(2019).