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DOI	10.1073/pnas.1808200116
	Escape band in Escherichia coli chemotaxis in opposing attractant and nutrient gradients
	Zhang X.; Si G.; Dong Y.; Chen K.; Ouyang Q.; Luo C.; Tu Y.
发表日期	2019
ISSN	0027-8424
起始页码	2253
结束页码	2258
卷号	116 期号:6
英文摘要	It is commonly believed that bacterial chemotaxis helps cells find food. However, not all attractants are nutrients, and not all nutrients are strong attractants. Here, by using microfluidic experiments, we studied Escherichia coli chemotaxis behavior in the presence of a strong chemoattractant (e.g., aspartate or methylaspartate) gradient and an opposing gradient of diluted tryptone broth (TB) growth medium. Our experiments showed that cells initially accumulate near the strong attractant source. However, after the peak cell density (h) reaches a critical value hc, the cells form a “escape band” (EB) that moves toward the chemotactically weaker but metabolically richer nutrient source. By using various mutant strains and varying experimental conditions, we showed that the competition between Tap and Tar receptors is the key molecular mechanism underlying the formation of the escape band. A mathematical model combining chemotaxis signaling and cell growth was developed to explain the experiments quantitatively. The model also predicted that the width w and the peak position xp of EB satisfy two scaling relations: w/l ∼ (h/hc)−1/ 2 and 1 − xp/l ∼ (h/hc)−1/ 2 , where l is the channel length. Both scaling relations were verified by experiments. Our study shows that the combination of nutrient consumption, population growth, and chemotaxis with multiple receptors allows cells to search for optimal growth condition in complex environments with conflicting sources. © 2019 National Academy of Sciences. All Rights Reserved.
英文关键词	Chemotaxis; Growth; Opposing gradients; Traveling wave
语种	英语
scopus关键词	aspartic acid; chemoattractant; methylaspartic acid; receptor; Tap receptor; Tar receptor; unclassified drug; chemotactic factor; Article; bacterial growth; bacterial phenomena and functions; bacterium mutant; broth dilution; cell density; chemotaxis; controlled study; critical value; escape band; Escherichia coli; mathematical model; microfluidics; nonhuman; nutrient; priority journal; quantitative analysis; algorithm; biological model; chemotaxis; Escherichia coli; immunology; metabolism; microfluidic analysis; physiology; reproducibility; Algorithms; Chemotactic Factors; Chemotaxis; Escherichia coli; Microfluidic Analytical Techniques; Models, Biological; Nutrients; Reproducibility of Results
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160404
作者单位	Zhang, X., State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, 100871, China, Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China; Si, G., State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, 100871, China, Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China; Dong, Y., Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China; Chen, K., State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, 100871, China; Ouyang, Q., State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing, 100871, China, Center for Quantitative Biology, Academy for ...
推荐引用方式 GB/T 7714	Zhang X.,Si G.,Dong Y.,et al. Escape band in Escherichia coli chemotaxis in opposing attractant and nutrient gradients[J],2019,116(6).
APA	Zhang X..,Si G..,Dong Y..,Chen K..,Ouyang Q..,...&Tu Y..(2019).Escape band in Escherichia coli chemotaxis in opposing attractant and nutrient gradients.Proceedings of the National Academy of Sciences of the United States of America,116(6).
MLA	Zhang X.,et al."Escape band in Escherichia coli chemotaxis in opposing attractant and nutrient gradients".Proceedings of the National Academy of Sciences of the United States of America 116.6(2019).