气候变化领域集成服务门户(CCPortal): Molecular mechanism and history of non-sense to sense evolution of antifreeze glycoprotein gene in northern gadids

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DOI	10.1073/pnas.1817138116
	Molecular mechanism and history of non-sense to sense evolution of antifreeze glycoprotein gene in northern gadids
	Zhuang X.; Yang C.; Murphy K.R.; Christina Cheng C.-H.
发表日期	2019
ISSN	0027-8424
起始页码	4400
结束页码	4405
卷号	116 期号:10
英文摘要	A fundamental question in evolutionary biology is how genetic novelty arises. De novo gene birth is a recently recognized mechanism, but the evolutionary process and function of putative de novo genes remain largely obscure. With a clear life-saving function, the diverse antifreeze proteins of polar fishes are exemplary adaptive innovations and models for investigating new gene evolution. Here, we report clear evidence and a detailed molecular mechanism for the de novo formation of the northern gadid (codfish) antifreeze glycoprotein (AFGP) gene from a minimal noncoding sequence. We constructed genomic DNA libraries for AFGP-bearing and AFGP-lacking species across the gadid phylogeny and performed fine-scale comparative analyses of the AFGP genomic loci and homologs. We identified the noncoding founder region and a nine-nucleotide (9-nt) element therein that supplied the codons for one Thr-Ala-Ala unit from which the extant repetitive AFGP-coding sequence (cds) arose through tandem duplications. The latent signal peptide (SP)-coding exons were fortuitous noncoding DNA sequence immediately upstream of the 9-nt element, which, when spliced, supplied a typical secretory signal. Through a 1-nt frameshift mutation, these two parts formed a single read-through open reading frame (ORF). It became functionalized when a putative translocation event conferred the essential cis promoter for transcriptional initiation. We experimentally proved that all genic components of the extant gadid AFGP originated from entirely nongenic DNA. The gadid AFGP evolutionary process also represents a rare example of the proto-ORF model of de novo gene birth where a fully formed ORF existed before the regulatory element to activate transcription was acquired. © 2019 National Academy of Sciences. All Rights Reserved.
英文关键词	Adaptive evolution; Codfish AFGP; De novo gene; Noncoding origin; Proto-ORF
语种	英语
scopus关键词	genomic DNA; AFGP gene; Article; comparative study; DNA library; DNA sequence; evidence based medicine; evolution; exon; fish; frameshift mutation; gene; gene locus; gene sequence; genetic code; genetic identification; nonhuman; northern gadid; open reading frame; phylogeny; priority journal; promoter region
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160390
作者单位	Zhuang, X., School of Integrative Biology, University of Illinois at Urbana–Champaign, Urbana, IL 61801, United States, Department of Ecology and Evolution, University of Chicago, Chicago, IL 60637, United States; Yang, C., School of Integrative Biology, University of Illinois at Urbana–Champaign, Urbana, IL 61801, United States; Murphy, K.R., School of Integrative Biology, University of Illinois at Urbana–Champaign, Urbana, IL 61801, United States; Christina Cheng, C.-H., School of Integrative Biology, University of Illinois at Urbana–Champaign, Urbana, IL 61801, United States
推荐引用方式 GB/T 7714	Zhuang X.,Yang C.,Murphy K.R.,et al. Molecular mechanism and history of non-sense to sense evolution of antifreeze glycoprotein gene in northern gadids[J],2019,116(10).
APA	Zhuang X.,Yang C.,Murphy K.R.,&Christina Cheng C.-H..(2019).Molecular mechanism and history of non-sense to sense evolution of antifreeze glycoprotein gene in northern gadids.Proceedings of the National Academy of Sciences of the United States of America,116(10).
MLA	Zhuang X.,et al."Molecular mechanism and history of non-sense to sense evolution of antifreeze glycoprotein gene in northern gadids".Proceedings of the National Academy of Sciences of the United States of America 116.10(2019).