气候变化领域集成服务门户(CCPortal): Ionic stress enhances ER–PM connectivity via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis

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DOI	10.1073/pnas.1818099116
	Ionic stress enhances ER–PM connectivity via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis
	Lee E.; Vanneste S.; Pérez-Sancho J.; Benitez-Fuente F.; Strelau M.; Macho A.P.; Botella M.A.; Friml J.; Rosado A.
发表日期	2019
ISSN	0027-8424
起始页码	1420
结束页码	1429
卷号	116 期号:4
英文摘要	The interorganelle communication mediated by membrane contact sites (MCSs) is an evolutionary hallmark of eukaryotic cells. MCS connections enable the nonvesicular exchange of information between organelles and allow them to coordinate responses to changing cellular environments. In plants, the importance of MCS components in the responses to environmental stress has been widely established, but the molecular mechanisms regulating interorganelle connectivity during stress still remain opaque. In this report, we use the model plant Arabidopsis thaliana to show that ionic stress increases endoplasmic reticulum (ER)–plasma membrane (PM) connectivity by promoting the cortical expansion of synaptotagmin 1 (SYT1)-enriched ER–PM contact sites (S-EPCSs). We define differential roles for the cortical cytoskeleton in the regulation of S-EPCS dynamics and ER–PM connectivity, and we identify the accumulation of phosphatidylinositol 4,5-bisphos-phate [PI(4,5)P 2 ] at the PM as a molecular signal associated with the ER–PM connectivity changes. Our study highlights the functional conservation of EPCS components and PM phosphoinositides as modulators of ER–PM connectivity in eukaryotes, and uncovers unique aspects of the spatiotemporal regulation of ER–PM connectivity in plants. © 2019 National Academy of Sciences. All Rights Reserved.
英文关键词	Cytoskeleton; ER–PM contact sites; Ionic stress; Phosphoinositides; SYT1
语种	英语
scopus关键词	phosphatidylinositide; phosphatidylinositol 4,5 bisphosphate; synaptotagmin I; Arabidopsis protein; phosphatidylinositol; phosphatidylinositol 4,5 bisphosphate; synaptotagmin I; SYT1 protein, Arabidopsis; Arabidopsis thaliana; Article; cell expansion; cell membrane; cell membrane contact site; controlled study; cytoskeleton; endoplasmic reticulum; eukaryote; ionic stress; nonhuman; plant stress; priority journal; signal transduction; Arabidopsis; cell membrane; endoplasmic reticulum; metabolism; physiological stress; physiology; Arabidopsis; Arabidopsis Proteins; Cell Membrane; Cytoskeleton; Endoplasmic Reticulum; Eukaryota; Phosphatidylinositol 4,5-Diphosphate; Phosphatidylinositols; Stress, Physiological; Synaptotagmin I
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160383
作者单位	Lee, E., Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, BC V6T 1Z4, Canada; Vanneste, S., Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, 9000, Belgium, Center for Plant Systems Biology, Vlaams Instituut voor Biotechnologie, Ghent, 9052, Belgium, Lab of Plant Growth Analysis, Ghent University Global Campus, Incheon, 21985, South Korea; Pérez-Sancho, J., Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga–Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Málaga, 29071, Spain, Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai, 201602, China; Benitez-Fuente, F., Department of Botany, Faculty of Sciences, University of British Columbia, Vancouver, BC V6T 1Z4, Canada, Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga...
推荐引用方式 GB/T 7714	Lee E.,Vanneste S.,Pérez-Sancho J.,等. Ionic stress enhances ER–PM connectivity via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis[J],2019,116(4).
APA	Lee E..,Vanneste S..,Pérez-Sancho J..,Benitez-Fuente F..,Strelau M..,...&Rosado A..(2019).Ionic stress enhances ER–PM connectivity via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America,116(4).
MLA	Lee E.,et al."Ionic stress enhances ER–PM connectivity via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis".Proceedings of the National Academy of Sciences of the United States of America 116.4(2019).