气候变化领域集成服务门户(CCPortal): Polar vacuolar distribution is essential for accurate asymmetric division of Arabidopsis zygotes

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DOI	10.1073/pnas.1814160116
	Polar vacuolar distribution is essential for accurate asymmetric division of Arabidopsis zygotes
	Kimata Y.; Kato T.; Higaki T.; Kurihara D.; Yamada T.; Segami S.; Morita M.T.; Maeshima M.; Hasezawa S.; Higashiyama T.; Tasaka M.; Ueda M.
发表日期	2019
ISSN	0027-8424
起始页码	2338
结束页码	2343
卷号	116 期号:6
英文摘要	In most flowering plants, the asymmetric cell division of the zygote is the initial step in establishing the apical-basal axis of the mature plant. The zygote is polarized, possessing the nucleus at the apical tip and large vacuoles at the basal end. Despite their known polar localization, whether the positioning of the vacuoles and the nucleus is coordinated and what the role of the vacuole is in the asymmetric zygotic division remain elusive. In the present study, we utilized a live-cell imaging system to visualize the dynamics of vacuoles during the entire process of zygote polarization in Arabidopsis. Image analysis revealed that the vacuoles formed tubular strands around the apically migrating nucleus. They gradually accumulated at the basal region and filled the space, resulting in asymmetric distribution in the mature zygote. To assess the role of vacuoles in the zygote, we screened various vacuole mutants and identified that shoot gravitropism2 (sgr2), in which the vacuolar structural change was impaired, failed to form tubular vacuoles and to polarly distribute the vacuole. In sgr2, large vacuoles occupied the apical tip and thus nuclear migration was blocked, resulting in a more symmetric zygotic division. We further observed that tubular vacuole formation and asymmetric vacuolar distribution both depended on the longitudinal array of actin filaments. Overall, our results show that vacuolar dynamics is crucial not only for the polar distribution along actin filaments but also for adequate nuclear positioning, and consequently zygote-division asymmetry. © 2019 National Academy of Sciences. All Rights Reserved.
英文关键词	Apical-basal axis; Arabidopsis thaliana; Live-cell imaging; Vacuole; Zygote
语种	英语
scopus关键词	actin filament; Arabidopsis; Article; cell structure; cell vacuole; cellular distribution; controlled study; embryo; image analysis; live cell imaging; nonhuman; nuclear localization signal; ontogeny; priority journal; shoot gravitropism2 gene; transgene; zygote; Arabidopsis; asymmetric cell division; cell nucleus; cell polarity; cell vacuole; cytology; fluorescent antibody technique; genetics; metabolism; mutation; physiology; zygote; Arabidopsis protein; chloroplast protein; SGR2 protein, Arabidopsis; Actin Cytoskeleton; Arabidopsis; Arabidopsis Proteins; Asymmetric Cell Division; Cell Nucleus; Cell Polarity; Chloroplast Proteins; Fluorescent Antibody Technique; Mutation; Vacuoles; Zygote
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160378
作者单位	Kimata, Y., Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602, Japan; Kato, T., Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630-0192, Japan; Higaki, T., Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwanoha, Kashiwa, Chiba, 277-8562, Japan, International Research Organization for Advanced Science and Technology, Kumamoto University, Chuo-ku, Kumamoto, 860-8555, Japan; Kurihara, D., Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602, Japan; Yamada, T., Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602, Japan, Institute of Transformative Bio-Molecules, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan; Segami, S., Graduate School of Bioagricultural Sciences, Nag...
推荐引用方式 GB/T 7714	Kimata Y.,Kato T.,Higaki T.,et al. Polar vacuolar distribution is essential for accurate asymmetric division of Arabidopsis zygotes[J],2019,116(6).
APA	Kimata Y..,Kato T..,Higaki T..,Kurihara D..,Yamada T..,...&Ueda M..(2019).Polar vacuolar distribution is essential for accurate asymmetric division of Arabidopsis zygotes.Proceedings of the National Academy of Sciences of the United States of America,116(6).
MLA	Kimata Y.,et al."Polar vacuolar distribution is essential for accurate asymmetric division of Arabidopsis zygotes".Proceedings of the National Academy of Sciences of the United States of America 116.6(2019).