气候变化领域集成服务门户(CCPortal): Arabidopsis ABCG28 is required for the apical accumulation of reactive oxygen species in growing pollen tubes

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DOI	10.1073/pnas.1902010116
	Arabidopsis ABCG28 is required for the apical accumulation of reactive oxygen species in growing pollen tubes
	Do T.H.T.; Choi H.; Palmgren M.; Martinoia E.; Hwang J.-U.; Lee Y.
发表日期	2019
ISSN	0027-8424
起始页码	12540
结束页码	12549
卷号	116 期号:25
英文摘要	Tip-focused accumulation of reactive oxygen species (ROS) is tightly associated with pollen tube growth and is thus critical for fertilization. However, it is unclear how tip-growing cells establish such specific ROS localization. Polyamines have been proposed to function in tip growth as precursors of the ROS, hydrogen peroxide. The ABC transporter AtABCG28 may regulate ROS status, as it contains multiple cysteine residues, a characteristic of proteins involved in ROS homeostasis. In this study, we found that AtABCG28 was specifically expressed in the mature pollen grains and pollen tubes. AtABCG28 was localized to secretory vesicles inside the pollen tube that moved toward and fused with the plasma membrane of the pollen tube tip. Knocking out AtABCG28 resulted in defective pollen tube growth, failure to localize polyamine and ROS to the growing pollen tube tip, and complete male sterility, whereas ectopic expression of this gene in root hair could recover ROS accumulation at the tip and improved the growth under high-pH conditions, which normally prevent ROS accumulation and tip growth. Together, these data suggest that AtABCG28 is critical for localizing polyamine and ROS at the growing tip. In addition, this function of AtABCG28 is likely to protect the pollen tube from the cytotoxicity of polyamine and contribute to the delivery of polyamine to the growing tip for incorporation into the expanding cell wall. © 2019 National Academy of Sciences. All rights reserved.
英文关键词	ABC transporters; AtABCG28; Pollen tube growth; Polyamine; ROS
语种	英语
scopus关键词	ABC transporter; polyamine; reactive oxygen metabolite; Arabidopsis thaliana; Article; AtABCG28 gene; cell membrane; cell migration; cell wall; controlled study; gene; gene expression; gene function; gene knockdown; gene location; germination; in vitro study; nonhuman; pollen tube; priority journal; secretory vesicle
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160362
作者单位	Do, T.H.T., Division of Integrative Bioscience and Biotechnology, POSTECH, Pohang, 37673, South Korea; Choi, H., Division of Integrative Bioscience and Biotechnology, POSTECH, Pohang, 37673, South Korea; Palmgren, M., Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, DK-1871, Denmark; Martinoia, E., Division of Integrative Bioscience and Biotechnology, POSTECH, Pohang, 37673, South Korea, Department of Plant and Microbial Biology, University of Zurich, Zurich, 8008, Switzerland; Hwang, J.-U., Division of Integrative Bioscience and Biotechnology, POSTECH, Pohang, 37673, South Korea; Lee, Y., Division of Integrative Bioscience and Biotechnology, POSTECH, Pohang, 37673, South Korea
推荐引用方式 GB/T 7714	Do T.H.T.,Choi H.,Palmgren M.,et al. Arabidopsis ABCG28 is required for the apical accumulation of reactive oxygen species in growing pollen tubes[J],2019,116(25).
APA	Do T.H.T.,Choi H.,Palmgren M.,Martinoia E.,Hwang J.-U.,&Lee Y..(2019).Arabidopsis ABCG28 is required for the apical accumulation of reactive oxygen species in growing pollen tubes.Proceedings of the National Academy of Sciences of the United States of America,116(25).
MLA	Do T.H.T.,et al."Arabidopsis ABCG28 is required for the apical accumulation of reactive oxygen species in growing pollen tubes".Proceedings of the National Academy of Sciences of the United States of America 116.25(2019).