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DOI10.1073/pnas.1905924116
A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae
Wang C.; Taki M.; Sato Y.; Tamura Y.; Yaginuma H.; Okada Y.; Yamaguchi S.
发表日期2019
ISSN0027-8424
起始页码15817
结束页码15822
卷号116期号:32
英文摘要Stimulation emission depletion (STED) microscopy enables ultrastructural imaging of organelle dynamics with a high spatiotemporal resolution in living cells. For the visualization of the mitochondrial membrane dynamics in STED microscopy, rationally designed mitochondrial fluorescent markers with enhanced photostability are required. Herein, we report the development of a superphotostable fluorescent labeling reagent with long fluorescence lifetime, whose design is based on a structurally reinforced naphthophosphole fluorophore that is conjugated with an electron-donating dipheny-lamino group. The combination of long-lived fluorescence and superphotostable features of the fluorophore allowed us to selectively capture the ultrastructures of the mitochondrial cristae with a resolution of ∼60 nm when depleted at 660 nm. This chemical tool provides morphological information of the cristae, which has so far only been observed in fixed cells using electron microscopy. Moreover, this method gives information about the dynamic ultrastructures such as the intermembrane fusion in different mitochondria as well as the intercristae mergence in a single mitochondrion during the apoptosis-like mitochondrial swelling process. © 2019 National Academy of Sciences. All rights reserved.
英文关键词Cristae; Fluorescence probe; Live-cell imaging; Mitochondrial; STED microscopy; Superresolution
语种英语
scopus关键词apoptosis; article; electron microscopy; fluorescence; live cell imaging; mitochondrion swelling; ultrastructure; cell line; chemistry; human; light; metabolism; mitochondrial membrane; mitochondrion; three-dimensional imaging; time lapse imaging; fluorescent dye; Cell Line; Fluorescent Dyes; Humans; Imaging, Three-Dimensional; Light; Mitochondria; Mitochondrial Membranes; Time-Lapse Imaging
来源期刊Proceedings of the National Academy of Sciences of the United States of America
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/160358
作者单位Wang, C., Institute of Transformative Bio-Molecules, Nagoya University, Nagoya, 464-8601, Japan; Taki, M., Institute of Transformative Bio-Molecules, Nagoya University, Nagoya, 464-8601, Japan, Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan; Sato, Y., Institute of Transformative Bio-Molecules, Nagoya University, Nagoya, 464-8601, Japan; Tamura, Y., Department of Material and Biological Chemistry, Faculty of Science, Yamagata University, Yamagata, 990‐8560, Japan; Yaginuma, H., Laboratory for Cell Polarity Regulation, Center for Biosystems Dynamics Research, RIKEN, Osaka, 565-0874, Japan; Okada, Y., Laboratory for Cell Polarity Regulation, Center for Biosystems Dynamics Research, RIKEN, Osaka, 565-0874, Japan, Department of Physics, Graduate School of Science, University of Tokyo, Tokyo, 113-0033, Japan, Universal Biology Institute, Graduate School of Science, University of Tokyo, Tokyo, 113-0033, Japan, Internatio...
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Wang C.,Taki M.,Sato Y.,et al. A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae[J],2019,116(32).
APA Wang C..,Taki M..,Sato Y..,Tamura Y..,Yaginuma H..,...&Yamaguchi S..(2019).A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae.Proceedings of the National Academy of Sciences of the United States of America,116(32).
MLA Wang C.,et al."A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae".Proceedings of the National Academy of Sciences of the United States of America 116.32(2019).
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