气候变化领域集成服务门户(CCPortal): Rickettsia conorii O antigen is the target of bactericidal Weil-Felix antibodies

CCPortal

DOI	10.1073/pnas.1911922116
	Rickettsia conorii O antigen is the target of bactericidal Weil-Felix antibodies
	Kim H.K.; Premaratna R.; Missiakas D.M.; Schneewind O.
发表日期	2019
ISSN	0027-8424
起始页码	19659
结束页码	19664
卷号	116 期号:39
英文摘要	Rickettsial diseases have long been diagnosed with serum antibodies cross-reactive against Proteus vulgaris (Weil-Felix reaction). Although Weil-Felix antibodies are associated with the development of immunity, their rickettsial target and contribution to disease pathogenesis are not established. Here, we developed a transposon for insertional mutagenesis of Rickettsia conorii, isolating variants defective for replication in cultured cells and in spotted fever pathogenesis. Mutations in the polysaccharide synthesis operon (pso) abolish lipopolysaccharide O-antigen synthesis and Weil-Felix serology and alter outer-membrane protein assembly. Unlike wild-type R. conorii, pso mutants cannot elicit bactericidal antibodies that bind O antigen. The pso operon is conserved among rickettsial pathogens, suggesting that bactericidal antibodies targeting O antigen may generate universal immunity that could be exploited to develop vaccines against rickettsial diseases. © 2019 National Academy of Sciences. All rights reserved.
英文关键词	Lipopolysaccharide; O antigen; Polysaccharide synthesis operon; Transposon mutagenesis; Weil-Felix reaction
语种	英语
scopus关键词	bacterium antibody; lipopolysaccharide; O antigen; outer membrane protein; polysaccharide; unclassified drug; Weil Felix antibody; Article; bacterial immunity; bacterial strain; bacterium isolation; controlled study; insertional mutagenesis; nonhuman; operon; priority journal; protein assembly; Rickettsia conorii; serology; transposon mutagenesis; wild type
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160345
作者单位	Kim, H.K., Howard Taylor Ricketts Laboratory, Argonne National Laboratory, Lemont, IL 60649, United States, Department of Microbiology, University of Chicago, Chicago, IL 60637, United States; Premaratna, R., Department of Medicine, Faculty of Medicine, University of Kelaniya, Ragama, 11010, Sri Lanka; Missiakas, D.M., Howard Taylor Ricketts Laboratory, Argonne National Laboratory, Lemont, IL 60649, United States, Department of Microbiology, University of Chicago, Chicago, IL 60637, United States; Schneewind, O., Howard Taylor Ricketts Laboratory, Argonne National Laboratory, Lemont, IL 60649, United States, Department of Microbiology, University of Chicago, Chicago, IL 60637, United States
推荐引用方式 GB/T 7714	Kim H.K.,Premaratna R.,Missiakas D.M.,et al. Rickettsia conorii O antigen is the target of bactericidal Weil-Felix antibodies[J],2019,116(39).
APA	Kim H.K.,Premaratna R.,Missiakas D.M.,&Schneewind O..(2019).Rickettsia conorii O antigen is the target of bactericidal Weil-Felix antibodies.Proceedings of the National Academy of Sciences of the United States of America,116(39).
MLA	Kim H.K.,et al."Rickettsia conorii O antigen is the target of bactericidal Weil-Felix antibodies".Proceedings of the National Academy of Sciences of the United States of America 116.39(2019).