Climate Change Data Portal
| DOI | 10.1073/pnas.2020857118 |
| Naphthylphthalamic acid associates with and inhibits PIN auxin transporters | |
| Abas L.; Kolb M.; Stadlmann J.; Janacek D.P.; Lukic K.; Schwechheimer C.; Sazanov L.A.; Mach L.; Friml J.; Hammes U.Z. | |
| 发表日期 | 2020 |
| ISSN | 0027-8424 |
| 卷号 | 118期号:1 |
| 英文摘要 | N-1-naphthylphthalamic acid (NPA) is a key inhibitor of directional (polar) transport of the hormone auxin in plants. For decades, it has been a pivotal tool in elucidating the unique polar auxin transportbased processes underlying plant growth and development. Its exact mode of action has long been sought after and is still being debated, with prevailing mechanistic schemes describing only indirect connections between NPA and the main transporters responsible for directional transport, namely PIN auxin exporters. Here we present data supporting a model in which NPA associates with PINs in a more direct manner than hitherto postulated. We show that NPA inhibits PIN activity in a heterologous oocyte system and that expression of NPA-sensitive PINs in plant, yeast, and oocyte membranes leads to specific saturable NPA binding. We thus propose that PINs are a bona fide NPA target. This offers a straightforward molecular basis for NPA inhibition of PIN-dependent auxin transport and a logical parsimonious explanation for the known physiological effects of NPA on plant growth, as well as an alternative hypothesis to interpret past and future results. We also introduce PIN dimerization and describe an effect of NPA on this, suggesting that NPA binding could be exploited to gain insights into structural aspects of PINs related to their transport mechanism. © 2021 National Academy of Sciences. All rights reserved. |
| 英文关键词 | Auxin transport; Auxin transport inhibitor; Naphthylphthalamic acid; NPA; PIN |
| 语种 | 英语 |
| 来源期刊 | Proceedings of the National Academy of Sciences of the United States of America
 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/160325 |
| 作者单位 | Abas, L., Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences (BOKU), Vienna, 1190, Austria; Kolb, M., Plant Systems Biology, School of Life Sciences Weihenstephan, Technical University of Munich, Freising, 85354, Germany; Stadlmann, J., Department of Chemistry, University of Natural Resources and Life Sciences (BOKU), Vienna, 1190, Austria; Janacek, D.P., Plant Systems Biology, School of Life Sciences Weihenstephan, Technical University of Munich, Freising, 85354, Germany; Lukic, K., Institute of Science and Technology Austria, Klosterneuburg, 3400, Austria; Schwechheimer, C., Plant Systems Biology, School of Life Sciences Weihenstephan, Technical University of Munich, Freising, 85354, Germany; Sazanov, L.A., Institute of Science and Technology Austria, Klosterneuburg, 3400, Austria; Mach, L., Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences (BOKU), Vienna, 1190, Austria; Friml, J., Institute of Science and T... |
| 推荐引用方式 GB/T 7714 | Abas L.,Kolb M.,Stadlmann J.,et al. Naphthylphthalamic acid associates with and inhibits PIN auxin transporters[J],2020,118(1). |
| APA | Abas L..,Kolb M..,Stadlmann J..,Janacek D.P..,Lukic K..,...&Hammes U.Z..(2020).Naphthylphthalamic acid associates with and inhibits PIN auxin transporters.Proceedings of the National Academy of Sciences of the United States of America,118(1). |
| MLA | Abas L.,et al."Naphthylphthalamic acid associates with and inhibits PIN auxin transporters".Proceedings of the National Academy of Sciences of the United States of America 118.1(2020). |
| 条目包含的文件 | 条目无相关文件。 | |||||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
