气候变化领域集成服务门户(CCPortal): The type IV pilin PilA couples surface attachment and cell-cycle initiation in Caulobacter crescentus

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DOI	10.1073/pnas.1920143117
	The type IV pilin PilA couples surface attachment and cell-cycle initiation in Caulobacter crescentus
	Medico L.D.; Cerletti D.; Schächle P.; Christen M.; Christen B.
发表日期	2020
ISSN	0027-8424
起始页码	9546
结束页码	9553
卷号	117 期号:17
英文摘要	Understanding how bacteria colonize surfaces and regulate cell-cycle progression in response to cellular adhesion is of fundamental importance. Here, we use transposon sequencing in conjunction with fluorescence resonance energy transfer (FRET) microscopy to uncover the molecular mechanism for how surface sensing drives cell-cycle initiation in Caulobacter crescentus. We identify the type IV pilin protein PilA as the primary signaling input that couples surface contact to cell-cycle initiation via the second messenger cyclic di-GMP (c-di-GMP). Upon retraction of pili filaments, the monomeric pilin reservoir in the inner membrane is sensed by the 17-amino acid transmembrane helix of PilA to activate the PleC-PleD two-component signaling system, increase cellular c-di-GMP levels, and signal the onset of the cell cycle. We termed the PilA signaling sequence CIP for “cell-cycle initiating pilin” peptide. Addition of the chemically synthesized CIP peptide initiates cell-cycle progression and simultaneously inhibits surface attachment. The broad conservation of the type IV pili and their importance in pathogens for host colonization suggests that CIP peptide mimetics offer strategies to inhibit surface sensing, prevent biofilm formation and control persistent infections. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	C-di-GMP; Caulobacter crescentus; FRET microscopy; TnSeq; Type IV pilin
语种	英语
scopus关键词	bacterial protein; cyclic GMP; monomer; periplasmic protein; pilin; PleC kinase; unclassified drug; fimbria protein; Article; bacterial colonization; Caulobacter vibrioides; cell cycle checkpoint; controlled study; fluorescence resonance energy transfer; gene cluster; gene expression regulation; gene identification; gene location; gene sequence; genetic susceptibility; inner membrane; membrane potential; molecular dynamics; nonhuman; periplasm; PilA gene; priority journal; protein synthesis; signal transduction; transposon; bacterium adherence; Caulobacter vibrioides; cell cycle; genetics; metabolism; physiology; Bacterial Adhesion; Caulobacter crescentus; Cell Cycle; Fimbriae Proteins; Gene Expression Regulation, Bacterial
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160296
作者单位	Medico, L.D., Institute of Molecular Systems Biology, Department of Biology, Eidgenössische Technische Hochschule Zürich, Zürich, 8093, Switzerland; Cerletti, D., Institute of Molecular Systems Biology, Department of Biology, Eidgenössische Technische Hochschule Zürich, Zürich, 8093, Switzerland; Schächle, P., Institute of Molecular Systems Biology, Department of Biology, Eidgenössische Technische Hochschule Zürich, Zürich, 8093, Switzerland; Christen, M., Institute of Molecular Systems Biology, Department of Biology, Eidgenössische Technische Hochschule Zürich, Zürich, 8093, Switzerland; Christen, B., Institute of Molecular Systems Biology, Department of Biology, Eidgenössische Technische Hochschule Zürich, Zürich, 8093, Switzerland
推荐引用方式 GB/T 7714	Medico L.D.,Cerletti D.,Schächle P.,et al. The type IV pilin PilA couples surface attachment and cell-cycle initiation in Caulobacter crescentus[J],2020,117(17).
APA	Medico L.D.,Cerletti D.,Schächle P.,Christen M.,&Christen B..(2020).The type IV pilin PilA couples surface attachment and cell-cycle initiation in Caulobacter crescentus.Proceedings of the National Academy of Sciences of the United States of America,117(17).
MLA	Medico L.D.,et al."The type IV pilin PilA couples surface attachment and cell-cycle initiation in Caulobacter crescentus".Proceedings of the National Academy of Sciences of the United States of America 117.17(2020).