气候变化领域集成服务门户(CCPortal): Global control of bacterial nitrogen and carbon metabolism by a PTSNtr-regulated switch

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DOI	10.1073/pnas.1917471117
	Global control of bacterial nitrogen and carbon metabolism by a PTSNtr-regulated switch
	Sánchez-Cañizares C.; Prell J.; Pini F.; Rutten P.; Kraxner K.; Wynands B.; Karunakaran R.; Poole P.S.
发表日期	2020
ISSN	0027-8424
起始页码	10234
结束页码	10245
卷号	117 期号:19
英文摘要	The nitrogen-related phosphotransferase system (PTSNtr) of Rhizobium leguminosarum bv. viciae 3841 transfers phosphate from PEP via PtsP and NPr to two output regulators, ManX and PtsN. ManX controls central carbon metabolism via the tricarboxylic acid (TCA) cycle, while PtsN controls nitrogen uptake, exopolysaccharide production, and potassium homeostasis, each of which is critical for cellular adaptation and survival. Cellular nitrogen status modulates phosphorylation when glutamine, an abundant amino acid when nitrogen is available, binds to the GAF sensory domain of PtsP, preventing PtsP phosphorylation and subsequent modification of ManX and PtsN. Under nitrogen-rich, carbon-limiting conditions, unphosphorylated ManX stimulates the TCA cycle and carbon oxidation, while unphosphorylated PtsN stimulates potassium uptake. The effects are reversed with the phosphorylation of ManX and PtsN, occurring under nitrogen-limiting, carbon-rich conditions; phosphorylated PtsN triggers uptake and nitrogen metabolism, the TCA cycle and carbon oxidation are decreased, while carbon-storage polymers such as surface polysaccharide are increased. Deleting the GAF domain from PtsP makes cells "blind" to the cellular nitrogen status. PTSNtr constitutes a switch through which carbon and nitrogen metabolism are rapidly, and reversibly, regulated by protein:protein interactions. PTSNtr is widely conserved in proteobacteria, highlighting its global importance. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Bacterial metabolism; Nitrogen; Plant-host interactions; Regulatory network
语种	英语
scopus关键词	exopolysaccharide; nitrogen related phosphotransferase system; phosphotransferase; tricarboxylic acid; unclassified drug; bacterial protein; carbon; nitrogen; phosphate; phosphoenolpyruvate sugar phosphotransferase; adaptation; Article; bacterial metabolism; bacterial phenomena and functions; bacterial strain; carbon metabolism; cell survival; controlled study; enzyme activity; gene regulatory network; homeostasis; nitrogen metabolism; nitrogen uptake; nonhuman; potassium transport; priority journal; protein interaction; protein modification; protein phosphorylation; Proteobacteria; Rhizobium leguminosarum; signal transduction; citric acid cycle; gene expression regulation; genetics; growth, development and aging; metabolism; phosphorylation; promoter region; Bacterial Proteins; Carbon; Citric Acid Cycle; Gene Expression Regulation, Bacterial; Nitrogen; Phosphates; Phosphoenolpyruvate Sugar Phosphotransferase System; Phosphorylation; Promoter Regions, Genetic; Rhizobium leguminosarum
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160292
作者单位	Sánchez-Cañizares, C., Department of Plant Sciences, University of Oxford, Oxford, OX1 3RB, United Kingdom; Prell, J., Soil Ecology, Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Aachen, D-52056, Germany; Pini, F., Department of Plant Sciences, University of Oxford, Oxford, OX1 3RB, United Kingdom; Rutten, P., Department of Plant Sciences, University of Oxford, Oxford, OX1 3RB, United Kingdom; Kraxner, K., Soil Ecology, Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Aachen, D-52056, Germany; Wynands, B., Soil Ecology, Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen, Aachen, D-52056, Germany; Karunakaran, R., Department of Molecular Microbiology, John Innes Centre, Norwich, NR4 7UH, United Kingdom; Poole, P.S., Department of Plant Sciences, University of Oxford, Oxford, OX1 3RB, United Kingdom
推荐引用方式 GB/T 7714	Sánchez-Cañizares C.,Prell J.,Pini F.,et al. Global control of bacterial nitrogen and carbon metabolism by a PTSNtr-regulated switch[J],2020,117(19).
APA	Sánchez-Cañizares C..,Prell J..,Pini F..,Rutten P..,Kraxner K..,...&Poole P.S..(2020).Global control of bacterial nitrogen and carbon metabolism by a PTSNtr-regulated switch.Proceedings of the National Academy of Sciences of the United States of America,117(19).
MLA	Sánchez-Cañizares C.,et al."Global control of bacterial nitrogen and carbon metabolism by a PTSNtr-regulated switch".Proceedings of the National Academy of Sciences of the United States of America 117.19(2020).