气候变化领域集成服务门户(CCPortal): Active forces shape the metaphase spindle through a mechanical instability

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DOI	10.1073/pnas.2002446117
	Active forces shape the metaphase spindle through a mechanical instability
	Oriola D.; Jülicher F.; Brugués J.
发表日期	2020
ISSN	0027-8424
起始页码	16154
结束页码	16159
卷号	117 期号:28
英文摘要	The metaphase spindle is a dynamic structure orchestrating chromosome segregation during cell division. Recently, soft matter approaches have shown that the spindle behaves as an active liquid crystal. Still, it remains unclear how active force generation contributes to its characteristic spindle-like shape. Here we combine theory and experiments to show that molecular motor-driven forces shape the structure through a barreling-type instability. We test our physical model by titrating dynein activity in Xenopus egg extract spindles and quantifying the shape and microtubule orientation. We conclude that spindles are shaped by the interplay between surface tension, nematic elasticity, and motor-driven active forces. Our study reveals how motor proteins can mold liquid crystalline droplets and has implications for the design of active soft materials. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Active matter; Dynein; Liquid crystals; Mitotic spindle; Xenopus laevis
语种	英语
scopus关键词	dynein adenosine triphosphatase; molecular motor; dynein adenosine triphosphatase; Xenopus protein; Article; controlled study; crystallization; elasticity; enzyme activity; genomic instability; metaphase; microtubule; molecular dynamics; nonhuman; priority journal; quantum theory; spindle cell; surface tension; titrimetry; Xenopus; animal; biomechanics; chemistry; drug effect; liquid crystal; metabolism; metaphase; mitosis; physiology; spindle apparatus; Xenopus laevis; Animals; Biomechanical Phenomena; Dyneins; Elasticity; Liquid Crystals; Metaphase; Microtubules; Mitosis; Spindle Apparatus; Surface Tension; Xenopus laevis; Xenopus Proteins
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160262
作者单位	Oriola, D., Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, 01307, Germany, Center for Systems Biology Dresden, Dresden, 01307, Germany, Max Planck Institute for the Physics of Complex Systems, Dresden, 01187, Germany, Cluster of Excellence Physics of Life, TU Dresden, Dresden, 01307, Germany; Jülicher, F., Center for Systems Biology Dresden, Dresden, 01307, Germany, Max Planck Institute for the Physics of Complex Systems, Dresden, 01187, Germany, Cluster of Excellence Physics of Life, TU Dresden, Dresden, 01307, Germany; Brugués, J., Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, 01307, Germany, Center for Systems Biology Dresden, Dresden, 01307, Germany, Max Planck Institute for the Physics of Complex Systems, Dresden, 01187, Germany, Cluster of Excellence Physics of Life, TU Dresden, Dresden, 01307, Germany
推荐引用方式 GB/T 7714	Oriola D.,Jülicher F.,Brugués J.. Active forces shape the metaphase spindle through a mechanical instability[J],2020,117(28).
APA	Oriola D.,Jülicher F.,&Brugués J..(2020).Active forces shape the metaphase spindle through a mechanical instability.Proceedings of the National Academy of Sciences of the United States of America,117(28).
MLA	Oriola D.,et al."Active forces shape the metaphase spindle through a mechanical instability".Proceedings of the National Academy of Sciences of the United States of America 117.28(2020).