气候变化领域集成服务门户(CCPortal): Rocks in the auxin stream: Wound-induced auxin accumulation and ERF115 expression synergistically drive stem cell regeneration

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DOI	10.1073/pnas.2006620117
	Rocks in the auxin stream: Wound-induced auxin accumulation and ERF115 expression synergistically drive stem cell regeneration
	Canher B.; Heyman J.; Savina M.; Devendran A.; Eekhout T.; Vercauteren I.; Prinsen E.; Matosevich R.; Xu J.; Mironova V.; de Veylder L.
发表日期	2020
ISSN	0027-8424
起始页码	16667
结束页码	16677
卷号	117 期号:28
英文摘要	Plants are known for their outstanding capacity to recover from various wounds and injuries. However, it remains largely unknown how plants sense diverse forms of injury and canalize existing developmental processes into the execution of a correct regenerative response. Auxin, a cardinal plant hormone with morphogen-like properties, has been previously implicated in the recovery from diverse types of wounding and organ loss. Here, through a combination of cellular imaging and in silico modeling, we demonstrate that vascular stem cell death obstructs the polar auxin flux, much alike rocks in a stream, and causes it to accumulate in the endodermis. This in turn grants the endodermal cells the capacity to undergo periclinal cell division to repopulate the vascular stem cell pool. Replenishment of the vasculature by the endodermis depends on the transcription factor ERF115, a wound-inducible regulator of stem cell division. Although not the primary inducer, auxin is required to maintain ERF115 expression. Conversely, ERF115 sensitizes cells to auxin by activating ARF5/MONOPTEROS, an auxin-responsive transcription factor involved in the global auxin response, tissue patterning, and organ formation. Together, the wound-induced auxin accumulation and ERF115 expression grant the endodermal cells stem cell activity. Our work provides a mechanistic model for wound-induced stem cell regeneration in which ERF115 acts as a wound-inducible stem cell organizer that interprets wound-induced auxin maxima. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Arabidopsis; Auxin; ERF115; Regeneration; Stem cells
语种	英语
scopus关键词	adenosine diphosphate ribosylation factor 5; auxin; transcription factor; transcription factor ERF115; unclassified drug; Arabidopsis protein; ERF115 protein, Arabidopsis; indoleacetic acid derivative; phytohormone; transcription factor; Article; cell activity; cell death; cell division; cell regeneration; computer model; controlled study; endoderm; endodermis; histogenesis; live cell imaging; nonhuman; organogenesis; priority journal; protein expression; protein transport; stem cell; Arabidopsis; cell self-renewal; cytology; gene expression regulation; genetics; metabolism; physiology; plant epidermis; regeneration; Arabidopsis; Arabidopsis Proteins; Cell Division; Cell Self Renewal; Gene Expression Regulation, Plant; Indoleacetic Acids; Plant Epidermis; Plant Growth Regulators; Regeneration; Transcription Factors
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160259
作者单位	Canher, B., Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, B-9052, Belgium, Center for Plant Systems Biology, VIB, Ghent, B-9052, Belgium; Heyman, J., Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, B-9052, Belgium, Center for Plant Systems Biology, VIB, Ghent, B-9052, Belgium; Savina, M., Department for Systems Biology, Institute of Cytology and Genetics SB RAS, Novosibirsk, 630090, Russian Federation, LCT & EB, Novosibirsk State University, Novosibirsk, 630090, Russian Federation; Devendran, A., Department of Biological Sciences, Centre for BioImaging Sciences, National University of Singapore, Singapore, 117543, Singapore; Eekhout, T., Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, B-9052, Belgium, Center for Plant Systems Biology, VIB, Ghent, B-9052, Belgium; Vercauteren, I., Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, B-9052, Belgium, Center for Plant Systems Biology, VIB,...
推荐引用方式 GB/T 7714	Canher B.,Heyman J.,Savina M.,et al. Rocks in the auxin stream: Wound-induced auxin accumulation and ERF115 expression synergistically drive stem cell regeneration[J],2020,117(28).
APA	Canher B..,Heyman J..,Savina M..,Devendran A..,Eekhout T..,...&de Veylder L..(2020).Rocks in the auxin stream: Wound-induced auxin accumulation and ERF115 expression synergistically drive stem cell regeneration.Proceedings of the National Academy of Sciences of the United States of America,117(28).
MLA	Canher B.,et al."Rocks in the auxin stream: Wound-induced auxin accumulation and ERF115 expression synergistically drive stem cell regeneration".Proceedings of the National Academy of Sciences of the United States of America 117.28(2020).