气候变化领域集成服务门户(CCPortal): On the enigmatic disappearance of Rauber’s layer

CCPortal

DOI	10.1073/pnas.2002008117
	On the enigmatic disappearance of Rauber’s layer
	van Leeuwen J.; Rawson P.; Berg D.K.; Wells D.N.; Pfeffer P.L.
发表日期	2020
ISSN	0027-8424
起始页码	16409
结束页码	16417
卷号	117 期号:28
英文摘要	The polar trophoblast overlays the epiblast in eutherian mammals and, depending on the species, has one of two different fates. It either remains a single-layered, thinning epithelium called “Rauber’s layer,” which soon disintegrates, or, alternatively, it keeps proliferating, contributing heavily to the population of differentiating, invasive trophoblast cells and, at least in mice, to the induction of gastrulation. While loss of the persistent polar trophoblast in mice leads to reduced induction of gastrulation, we show here that prevention of the loss of the polar trophoblast in cattle results in ectopic domains of the gastrulation marker, BRACHYURY. This phenotype, and increased epiblast proliferation, arose when Rauber’s layer was maintained for a day longer by countering apoptosis through BCL2 overexpression. This suggests that the disappearance of Rauber’s layer is a necessity, presumably to avoid excessive signaling interactions between this layer and the subjacent epiblast. We note that, in all species in which the polar trophoblast persists, including humans and mice, ectopic polar trophoblast signaling is prevented via epiblast cavitation which leads to the (pro)amniotic cavity, whose function is to distance the central epiblast from such signaling interactions. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Amniotic cavity; Epiblast; Polar trophoblast
语种	英语
scopus关键词	beta galactosidase; bone morphogenetic protein; caspase 3; histone H3; protein bcl 2; protein Nodal; transcription factor; transcription factor brachyury; unclassified drug; Wnt protein; Brachyury protein; fetoprotein; protein bcl 2; T box transcription factor; amnion; animal cell; apoptosis; Article; bovine; cell differentiation; cell interaction; cell proliferation; controlled study; ectoderm; embryo; embryo development; gastrulation; gene overexpression; nonhuman; phenotype; placental mammal; priority journal; protein expression; Rauber layer; signal transduction; trophoblast; animal; cytology; embryology; female; genetics; germ layer; metabolism; mouse; pathophysiology; trophoblast; Animals; Apoptosis; Cattle; Cell Differentiation; Cell Proliferation; Female; Fetal Proteins; Gastrulation; Germ Layers; Mice; Proto-Oncogene Proteins c-bcl-2; T-Box Domain Proteins; Trophoblasts
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160258
作者单位	van Leeuwen, J., Animal Reproduction Group, Agresearch Ruakura, Hamilton, 3214, New Zealand, Department of Biomedical, Molecular and Cellular Biology, Waikato University, Hamilton, 3240, New Zealand; Rawson, P., School of Biological Sciences, Victoria University of Wellington, Wellington, 6012, New Zealand; Berg, D.K., Animal Reproduction Group, Agresearch Ruakura, Hamilton, 3214, New Zealand; Wells, D.N., Animal Reproduction Group, Agresearch Ruakura, Hamilton, 3214, New Zealand; Pfeffer, P.L., Animal Reproduction Group, Agresearch Ruakura, Hamilton, 3214, New Zealand, School of Biological Sciences, Victoria University of Wellington, Wellington, 6012, New Zealand
推荐引用方式 GB/T 7714	van Leeuwen J.,Rawson P.,Berg D.K.,等. On the enigmatic disappearance of Rauber’s layer[J],2020,117(28).
APA	van Leeuwen J.,Rawson P.,Berg D.K.,Wells D.N.,&Pfeffer P.L..(2020).On the enigmatic disappearance of Rauber’s layer.Proceedings of the National Academy of Sciences of the United States of America,117(28).
MLA	van Leeuwen J.,et al."On the enigmatic disappearance of Rauber’s layer".Proceedings of the National Academy of Sciences of the United States of America 117.28(2020).