气候变化领域集成服务门户(CCPortal): CAMSAP1 breaks the homeostatic microtubule network to instruct neuronal polarity

CCPortal

DOI	10.1073/pnas.1913177117
	CAMSAP1 breaks the homeostatic microtubule network to instruct neuronal polarity
	Zhou Z.; Xu H.; Li Y.; Yang M.; Zhang R.; Shiraishi A.; Kiyonari H.; Liang X.; Huang X.; Wang Y.; Xie Q.; Liu S.; Chen R.; Bao L.; Guo W.; Wang Y.; Meng W.
发表日期	2020
ISSN	0027-8424
起始页码	22193
结束页码	22203
卷号	117 期号:36
英文摘要	The establishment of axon/dendrite polarity is fundamental for neurons to integrate into functional circuits, and this process is critically dependent on microtubules (MTs). In the early stages of the establishment process, MTs in axons change dramatically with the morphological building of neurons; however, how the MT network changes are triggered is unclear. Here we showthat CAMSAP1 plays a decisive role in the neuronal axon identification process by regulating the number of MTs. Neurons lacking CAMSAP1 form a multiple axon phenotype in vitro, while the multipolar-bipolar transition and radialmigration are blocked in vivo.We demonstrate that the polarity regulator MARK2 kinase phosphorylates CAMSAP1 and affects its ability to bind to MTs, which in turn changes the protection of MT minus-ends and also triggers asymmetric distribution of MTs. Our results indicate that the polarized MT network in neurons is a decisive factor in establishing axon/dendritic polarity and is initially triggered by polarized signals. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	CAMSAP1; Cell migration; MARK2; Neuronal polarity; Noncentrosomal microtubules
语种	英语
scopus关键词	animal cell; animal experiment; animal tissue; Article; axon; CAMSAP1 gene; cell differentiation; cell polarity; controlled study; gene; homeostasis; in vitro study; in vivo study; microtubule; mouse; nonhuman; phenotype; priority journal; animal; cell polarity; drug effect; gene expression regulation; genetics; immunoprecipitation; metabolism; microtubule; nerve cell; physiology; Camsap1 protein, mouse; microtubule associated protein; paclitaxel; protein binding; Animals; Cell Polarity; Gene Expression Regulation; Immunoprecipitation; Mice; Microtubule-Associated Proteins; Microtubules; Neurons; Paclitaxel; Protein Binding
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160224
作者单位	Zhou, Z., State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 10019, China, College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China; Xu, H., State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 10019, China; Li, Y., State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 10019, China, College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China; Yang, M., State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 10019, China, Colle...
推荐引用方式 GB/T 7714	Zhou Z.,Xu H.,Li Y.,et al. CAMSAP1 breaks the homeostatic microtubule network to instruct neuronal polarity[J],2020,117(36).
APA	Zhou Z..,Xu H..,Li Y..,Yang M..,Zhang R..,...&Meng W..(2020).CAMSAP1 breaks the homeostatic microtubule network to instruct neuronal polarity.Proceedings of the National Academy of Sciences of the United States of America,117(36).
MLA	Zhou Z.,et al."CAMSAP1 breaks the homeostatic microtubule network to instruct neuronal polarity".Proceedings of the National Academy of Sciences of the United States of America 117.36(2020).