气候变化领域集成服务门户(CCPortal): The phosphatidylethanolamine-binding protein DTH1 mediates degradation of lipid droplets in Chlamydomonas reinhardtii

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DOI	10.1073/pnas.2005600117
	The phosphatidylethanolamine-binding protein DTH1 mediates degradation of lipid droplets in Chlamydomonas reinhardtii
	Lee J.; Yamaoka Y.; Kong F.; Cagnon C.; Beyly-Adriano A.; Jang S.; Gao P.; Kang B.-H.; Li-Beisson Y.; Lee Y.
发表日期	2020
ISSN	0027-8424
起始页码	23131
结束页码	23139
卷号	117 期号:37
英文摘要	Lipid droplets (LDs) are intracellular organelles found in a wide range of organisms and play important roles in stress tolerance. During nitrogen (N) starvation, Chlamydomonas reinhardtii stores large amounts of triacylglycerols (TAGs) inside LDs. When N is resupplied, the LDs disappear and the TAGs are degraded, presumably providing carbon and energy for regrowth. The mechanism by which cells degrade LDs is poorly understood. Here, we isolated a mutant (dth1-1, Delayed in TAG Hydrolysis 1) in which TAG degradation during recovery from N starvation was compromised. Consequently, the dth1-1 mutant grew poorly compared to its parental line during N recovery. Two additional independent loss-of-function mutants (dth1-2 and dth1-3) also exhibited delayed TAG remobilization. DTH1 transcript levels increased sevenfold upon N resupply, and DTH1 protein was localized to LDs. DTH1 contains a putative lipid-binding domain (DTH1LBD) with alpha helices predicted to be structurally similar to those in apolipoproteins E and A–I. Recombinant DTH1LBD bound specifically to phosphatidylethanolamine (PE), a major phospholipid coating the LD surface. Overexpression of DTH1LBD in Chlamydomonas phenocopied the dth1 mutant’s defective TAG degradation, suggesting that the function of DTH1 depends on its ability to bind PE. Together, our results demonstrate that the lipid-binding DTH1 plays an essential role in LD degradation and provide insight into the molecular mechanism of protein anchorage to LDs at the LD surface in photosynthetic cells. © 2020 National Academy of Sciences. All rights reserved.
语种	英语
scopus关键词	apolipoprotein A1; apolipoprotein E; binding protein; DTH1 protein; fat droplet; phosphatidylethanolamine; recombinant protein; triacylglycerol; unclassified drug; algal protein; fat droplet; nitrogen; phosphatidylethanolamine binding protein; phospholipid; triacylglycerol; algal growth; alpha helix; Article; Chlamydomonas reinhardtii; controlled study; dth1 1 gene; dth1 2 gene; dth1 3 gene; DTH1 gene; gene; gene overexpression; genetic transcription; lipid metabolism; loss of function mutation; mutant; nonhuman; photosynthesis; priority journal; protein binding; protein domain; protein function; protein localization; amino acid sequence; Chlamydomonas reinhardtii; lipid metabolism; metabolism; physiology; Algal Proteins; Amino Acid Sequence; Chlamydomonas reinhardtii; Lipid Droplets; Lipid Metabolism; Nitrogen; Phosphatidylethanolamine Binding Protein; Phospholipids; Photosynthesis; Triglycerides
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160223
作者单位	Lee, J., Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, 37673, South Korea; Yamaoka, Y., Department of Life Sciences, Pohang University of Science and Technology, Pohang, 37673, South Korea; Kong, F., Laboratory of Marine Biotechnology, School of Bioengineering, Dalian University of Technology, Dalian, 116024, China; Cagnon, C., Aix-Marseille University, Commission for Atomic Energy and Alternative Energies (CEA), CNRS, Institut de Biosciences et Biotechnologies Aix-Marseille (BIAM), CEA Cadarache, Saint Paul-Lez-Durance, 13108, France; Beyly-Adriano, A., Aix-Marseille University, Commission for Atomic Energy and Alternative Energies (CEA), CNRS, Institut de Biosciences et Biotechnologies Aix-Marseille (BIAM), CEA Cadarache, Saint Paul-Lez-Durance, 13108, France; Jang, S., Department of Life Sciences, Pohang University of Science and Technology, Pohang, 37673, South Korea; Gao, P., State Key Laboratory of Agrobiotechnology, School of Life Scienc...
推荐引用方式 GB/T 7714	Lee J.,Yamaoka Y.,Kong F.,et al. The phosphatidylethanolamine-binding protein DTH1 mediates degradation of lipid droplets in Chlamydomonas reinhardtii[J],2020,117(37).
APA	Lee J..,Yamaoka Y..,Kong F..,Cagnon C..,Beyly-Adriano A..,...&Lee Y..(2020).The phosphatidylethanolamine-binding protein DTH1 mediates degradation of lipid droplets in Chlamydomonas reinhardtii.Proceedings of the National Academy of Sciences of the United States of America,117(37).
MLA	Lee J.,et al."The phosphatidylethanolamine-binding protein DTH1 mediates degradation of lipid droplets in Chlamydomonas reinhardtii".Proceedings of the National Academy of Sciences of the United States of America 117.37(2020).