气候变化领域集成服务门户(CCPortal): EXO70D isoforms mediate selective autophagic degradation of type-A ARR proteins to regulate cytokinin sensitivity

CCPortal

DOI	10.1073/pnas.2013161117
	EXO70D isoforms mediate selective autophagic degradation of type-A ARR proteins to regulate cytokinin sensitivity
	Acheampong A.K.; Shanks C.; Cheng C.-Y.; Eric Schaller G.; Dagdas Y.; Kieber J.J.
发表日期	2020
ISSN	0027-8424
起始页码	27034
结束页码	27043
卷号	117 期号:43
英文摘要	The phytohormone cytokinin influences many aspects of plant growth and development, several of which also involve the cellular process of autophagy, including leaf senescence, nutrient remobilization, and developmental transitions. The Arabidopsis type-A response regulators (type-A ARR) are negative regulators of cytokinin signaling that are transcriptionally induced in response to cytokinin. Here, we describe a mechanistic link between cytokinin signaling and autophagy, demonstrating that plants modulate cytokinin sensitivity through autophagic regulation of type-A ARR proteins. Type-A ARR proteins were degraded by autophagy in an AUTOPHAGY-RELATED (ATG)5-dependent manner, and this degradation is promoted by phosphorylation on a conserved aspartate in the receiver domain of the type-A ARRs. EXO70D family members interacted with type-A ARR proteins, likely in a phosphorylation-dependent manner, and recruited them to autophagosomes via interaction of the EXO70D AIM with the core autophagy protein, ATG8. Consistently, loss-of-function exo70D1,2,3 mutants exhibited compromised targeting of type-A ARRs to autophagic vesicles, have elevated levels of type-A ARR proteins, and are hyposensitive to cytokinin. Disruption of both type-A ARRs and EXO70D1,2,3 compromised survival in carbon-deficient conditions, suggesting interaction between autophagy and cytokinin responsiveness in response to stress. These results indicate that the EXO70D proteins act as selective autophagy receptors to target type-A ARR cargos for autophagic degradation, demonstrating modulation of cytokinin signaling by selective autophagy. © 2020 National Academy of Sciences. All rights reserved.
英文关键词	Carbon starvation; Concanamycin A; Cytokinin signaling; Selective autophagy
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160204
作者单位	Acheampong, A.K., Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States; Shanks, C., Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States; Cheng, C.-Y., Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States; Eric Schaller, G., Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, United States; Dagdas, Y., Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, 1030, Austria; Kieber, J.J., Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States
推荐引用方式 GB/T 7714	Acheampong A.K.,Shanks C.,Cheng C.-Y.,et al. EXO70D isoforms mediate selective autophagic degradation of type-A ARR proteins to regulate cytokinin sensitivity[J],2020,117(43).
APA	Acheampong A.K.,Shanks C.,Cheng C.-Y.,Eric Schaller G.,Dagdas Y.,&Kieber J.J..(2020).EXO70D isoforms mediate selective autophagic degradation of type-A ARR proteins to regulate cytokinin sensitivity.Proceedings of the National Academy of Sciences of the United States of America,117(43).
MLA	Acheampong A.K.,et al."EXO70D isoforms mediate selective autophagic degradation of type-A ARR proteins to regulate cytokinin sensitivity".Proceedings of the National Academy of Sciences of the United States of America 117.43(2020).