气候变化领域集成服务门户(CCPortal): Control of septum thickness by the curvature of sepf polymers

CCPortal

DOI	10.1073/pnas.2002635118
	Control of septum thickness by the curvature of sepf polymers
	Wenzel M.; Celik Gulsoy I.N.; Gao Y.; Teng Z.; Willemse J.; Middelkamp M.; van Rosmalen M.G.M.; Larsen P.W.B.; van der Wel N.N.; Wuite G.J.L.; Roos W.H.; Hamoen L.W.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:2
英文摘要	Gram-positive bacteria divide by forming a thick cross wall. How the thickness of this septal wall is controlled is unknown. In this type of bacteria, the key cell division protein FtsZ is anchored to the cell membrane by two proteins, FtsA and/or SepF. We have isolated SepF homologs from different bacterial species and found that they all polymerize into large protein rings with diameters varying from 19 to 44 nm. Interestingly, these values correlated well with the thickness of their septa. To test whether ring diameter determines septal thickness, we tried to construct different SepF chimeras with the purpose to manipulate the diameter of the SepF protein ring. This was indeed possible and confirmed that the conserved core domain of SepF regulates ring diameter. Importantly, when SepF chimeras with different diameters were expressed in the bacterial host Bacillus subtilis, the thickness of its septa changed accordingly. These results strongly support a model in which septal thickness is controlled by curved molecular clamps formed by SepF polymers attached to the leading edge of nascent septa. This also implies that the intrinsic shape of a protein polymer can function as a mold to shape the cell wall. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Bacillus subtilis; Cell division; FtsZ; SepF
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/160182
作者单位	Wenzel, M., Bacterial Cell Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, 1098 XH, Netherlands; Celik Gulsoy, I.N., Bacterial Cell Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, 1098 XH, Netherlands; Gao, Y., Bacterial Cell Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, 1098 XH, Netherlands; Teng, Z., Bacterial Cell Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, 1098 XH, Netherlands; Willemse, J., Molecular Biotechnology, Institute of Biology, Leiden University, Leiden, 2333 BE, Netherlands; Middelkamp, M., Molecular Biophysics, Zernike Institute, University of Groningen, Groningen, 9747 AG, Netherlands; van Rosmalen, M.G.M., Department of Physics and Astronomy, Laser Lab., Free University of Amsterdam, Amsterdam, 1081 HV, Netherlands; Larsen, P.W.B., Department of Medical Biology, Electron Microscopy Center Amsterdam, Amsterdam UMC, Amsterdam, 1105 AZ, Ne...
推荐引用方式 GB/T 7714	Wenzel M.,Celik Gulsoy I.N.,Gao Y.,et al. Control of septum thickness by the curvature of sepf polymers[J],2021,118(2).
APA	Wenzel M..,Celik Gulsoy I.N..,Gao Y..,Teng Z..,Willemse J..,...&Hamoen L.W..(2021).Control of septum thickness by the curvature of sepf polymers.Proceedings of the National Academy of Sciences of the United States of America,118(2).
MLA	Wenzel M.,et al."Control of septum thickness by the curvature of sepf polymers".Proceedings of the National Academy of Sciences of the United States of America 118.2(2021).