气候变化领域集成服务门户(CCPortal): Diffusion-controlled metabolism for long-term survival of single isolated microorganisms trapped within ice crystals

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DOI	10.1073/pnas.0708183104
	Diffusion-controlled metabolism for long-term survival of single isolated microorganisms trapped within ice crystals
	Rohde R.A.; Price P.B.
发表日期	2007
ISSN	0027-8424
起始页码	16592
结束页码	16597
卷号	104 期号:42
英文摘要	Two known habitats for microbial metabolism in ice are surfaces of mineral grains and liquid veins along three-grain boundaries. We propose a third, more general, habitat in which a microbe frozen in ice can metabolize by redox reactions with dissolved small molecules such as CO2, O2, N2, CO, and CH4 diffusing through the ice lattice. We show that there is an adequate supply of diffusing molecules throughout deep glacial ice to sustain metabolism for >105 yr. Using scanning fluorimetry to map proteins (a proxy for cells) and F420 (a proxy for methanogens) in ice cores, we find isolated spikes of fluorescence with intensity consistent with as few as one microbial cell in a volume of 0.16 μl with the protein mapper and in 1.9 μl with the methanogen mapper. With such precise localization, it should be possible to extract single cells for molecular identification. © 2007 by The National Academy of Sciences of the USA.
英文关键词	Dissolved molecules; F420 fluorescence; Fluorimetry; Ice cores; Single cells
语种	英语
scopus关键词	article; cell isolation; diffusion; fluorescence; fluorometry; grain; mathematical computing; microbial activity; microbial growth; microorganism; nonhuman; oxidation reduction reaction; priority journal; roentgen spectroscopy; scanning electron microscopy
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/159178
作者单位	Rohde, R.A., Department of Physics, University of California, Berkeley, CA 94720, United States; Price, P.B., Department of Physics, University of California, Berkeley, CA 94720, United States
推荐引用方式 GB/T 7714	Rohde R.A.,Price P.B.. Diffusion-controlled metabolism for long-term survival of single isolated microorganisms trapped within ice crystals[J],2007,104(42).
APA	Rohde R.A.,&Price P.B..(2007).Diffusion-controlled metabolism for long-term survival of single isolated microorganisms trapped within ice crystals.Proceedings of the National Academy of Sciences of the United States of America,104(42).
MLA	Rohde R.A.,et al."Diffusion-controlled metabolism for long-term survival of single isolated microorganisms trapped within ice crystals".Proceedings of the National Academy of Sciences of the United States of America 104.42(2007).