气候变化领域集成服务门户(CCPortal): Ancient bacteria show evidence of DNA repair

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DOI	10.1073/pnas.0706787104
	Ancient bacteria show evidence of DNA repair
	Johnson S.S.; Hebsgaard M.B.; Christensen T.R.; Mastepanov M.; Nielsen R.; Munch K.; Brand T.; Gilbert M.T.P.; Zuber M.T.; Bunce M.; Rønn R.; Gilichinsky D.; Froese D.; Willerslev E.
发表日期	2007
ISSN	0027-8424
起始页码	14401
结束页码	14405
卷号	104 期号:36
英文摘要	Recent claims of cultivable ancient bacteria within sealed environments highlight our limited understanding of the mechanisms behind long-term cell survival. It remains unclear how dormancy, a favored explanation for extended cellular persistence, can cope with spontaneous genomic decay over geological timescales. There has been no direct evidence in ancient microbes for the most likely mechanism, active DNA repair, or for the metabolic activity necessary to sustain it. In this paper, we couple PCR and enzymatic treatment of DNA with direct respiration measurements to investigate long-term survival of bacteria sealed in frozen conditions for up to one million years. Our results show evidence of bacterial survival in samples up to half a million years in age, making this the oldest independently authenticated DNA to date obtained from viable cells. Additionally, we find strong evidence that this long-term survival is closely tied to cellular metabolic activity and DNA repair that over time proves to be superior to dormancy as a mechanism in sustaining bacteria viability. © 2007 by The National Academy of Sciences of the USA.
英文关键词	DNA damage; Long-term microbial survival; Metabolic activity
语种	英语
scopus关键词	bacterial DNA; carbon dioxide; nucleotide; ribosome DNA; article; bacterial cell; bacterial metabolism; bacterium; cell survival; cell viability; DNA damage; DNA repair; DNA sequence; molecular cloning; nonhuman; nucleotide sequence; polymerase chain reaction; priority journal; unindexed sequence; bacterium; gene amplification; genetics; microbiology; molecular genetics; nucleotide sequence; Bacteria (microorganisms); Bacteria; Base Sequence; DNA Repair; DNA, Bacterial; Gene Amplification; Molecular Sequence Data; Soil Microbiology
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/159010
作者单位	Johnson, S.S., Department of Earth, Atmospheric, and Planetary Sciences, Massachusetts Institute of Technology, 77 Massachusetts Avenue, 54-810, Cambridge, MA 02139, United States, Centre for Ancient Genetics, Institute of Biology, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen, Denmark; Hebsgaard, M.B., Centre for Ancient Genetics, Institute of Biology, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen, Denmark; Christensen, T.R., GeoBiosphere Science Centre, Lund University, Sölvegatan 12, Lund, 22362, Sweden; Mastepanov, M., GeoBiosphere Science Centre, Lund University, Sölvegatan 12, Lund, 22362, Sweden; Nielsen, R., Centre for Ancient Genetics, Institute of Biology, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen, Denmark; Munch, K., Centre for Ancient Genetics, Institute of Biology, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen, Denmark; Brand, T., Centre for Ancient Genetics, Institute of Biology, University of Copenha...
推荐引用方式 GB/T 7714	Johnson S.S.,Hebsgaard M.B.,Christensen T.R.,et al. Ancient bacteria show evidence of DNA repair[J],2007,104(36).
APA	Johnson S.S..,Hebsgaard M.B..,Christensen T.R..,Mastepanov M..,Nielsen R..,...&Willerslev E..(2007).Ancient bacteria show evidence of DNA repair.Proceedings of the National Academy of Sciences of the United States of America,104(36).
MLA	Johnson S.S.,et al."Ancient bacteria show evidence of DNA repair".Proceedings of the National Academy of Sciences of the United States of America 104.36(2007).