气候变化领域集成服务门户(CCPortal): Model metabolic strategy for heterotrophic bacteria in the cold ocean based on Colwellia psychrerythraea 34H

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DOI	10.1073/pnas.1807804115
	Model metabolic strategy for heterotrophic bacteria in the cold ocean based on Colwellia psychrerythraea 34H
	Czajka J.J.; Abernathy M.H.; Benites V.T.; Baidoo E.E.K.; Deming J.W.; Tang Y.J.
发表日期	2018
ISSN	0027-8424
起始页码	12507
结束页码	12512
卷号	115 期号:49
英文摘要	Colwellia psychrerythraea 34H is a model psychrophilic bacterium found in the cold ocean—polar sediments, sea ice, and the deep sea. Although the genomes of such psychrophiles have been sequenced, their metabolic strategies at low temperature have not been quantified. We measured the metabolic fluxes and gene expression of 34H at 4 °C (the mean global-ocean temperature and a normal-growth temperature for 34H), making comparative analyses at room temperature (above its upper-growth temperature of 18 °C) and with mesophilic Escherichia coli. When grown at 4 °C, 34H utilized multiple carbon substrates without catabolite repression or overflow byproducts; its anaplerotic pathways increased flux network flexibility and enabled CO2 fixation. In glucose-only medium, the Entner–Doudoroff (ED) pathway was the primary glycolytic route; in lactate-only medium, gluconeogenesis and the glyoxylate shunt became active. In comparison, E. coli, cold stressed at 4 °C, had rapid glycolytic fluxes but no biomass synthesis. At their respective normal-growth temperatures, intracellular concentrations of TCA cycle metabolites (α-ketoglutarate, succinate, malate) were 4–17 times higher in 34H than in E. coli, while levels of energy molecules (ATP, NADH, NADPH) were 10- to 100-fold lower. Experiments with E. coli mutants supported the thermodynamic advantage of the ED pathway at cold temperature. Heat-stressed 34H at room temperature (2 hours) revealed significant down-regulation of genes associated with glycolytic enzymes and flagella, while 24 hours at room temperature caused irreversible cellular damage. We suggest that marine heterotrophic bacteria in general may rely upon simplified metabolic strategies to overcome thermodynamic constraints and thrive in the cold ocean. © 2018 National Academy of Sciences. All rights reserved.
英文关键词	Acids; ED pathway; Gluconeogensis; Marine psychrophile; Metabolic flux; Short-chain fatty
语种	英语
scopus关键词	2 oxoglutaric acid; adenosine triphosphate; glycolytic enzyme; glyoxylic acid; malic acid; reduced nicotinamide adenine dinucleotide; reduced nicotinamide adenine dinucleotide phosphate; succinic acid; Article; bacterial growth; bacterial metabolism; biomass; bioremediation; carbon dioxide fixation; catabolite repression; cell culture; cell damage; citric acid cycle; cold stress; Colwellia psychrerythraea 34H; concentration (parameters); controlled study; down regulation; Escherichia coli; extremophile; flagellum; gene expression; genetic association; gluconeogenesis; glycolysis; greenhouse effect; growth curve; heat stress; heterotrophy; liquid chromatography-mass spectrometry; mass fragmentography; metabolic flux analysis; nonhuman; priority journal; RNA sequence; room temperature; sea ice; thermodynamics; water temperature; Alteromonadaceae; biological model; cold; energy metabolism; heterotrophy; metabolism; physiology; sea; Alteromonadaceae; Cold Temperature; Energy Metabolism; Heterotrophic Processes; Models, Biological; Oceans and Seas
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/158876
作者单位	Czajka, J.J., Department of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, MO 63130, United States; Abernathy, M.H., Department of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, MO 63130, United States; Benites, V.T., Technology Division, Joint BioEnergy Institute, Emeryville, CA 94608, United States, Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States; Baidoo, E.E.K., Technology Division, Joint BioEnergy Institute, Emeryville, CA 94608, United States, Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States; Deming, J.W., School of Oceanography, University of Washington, Seattle, WA 98105, United States; Tang, Y.J., Department of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, MO 63130, United States
推荐引用方式 GB/T 7714	Czajka J.J.,Abernathy M.H.,Benites V.T.,et al. Model metabolic strategy for heterotrophic bacteria in the cold ocean based on Colwellia psychrerythraea 34H[J],2018,115(49).
APA	Czajka J.J.,Abernathy M.H.,Benites V.T.,Baidoo E.E.K.,Deming J.W.,&Tang Y.J..(2018).Model metabolic strategy for heterotrophic bacteria in the cold ocean based on Colwellia psychrerythraea 34H.Proceedings of the National Academy of Sciences of the United States of America,115(49).
MLA	Czajka J.J.,et al."Model metabolic strategy for heterotrophic bacteria in the cold ocean based on Colwellia psychrerythraea 34H".Proceedings of the National Academy of Sciences of the United States of America 115.49(2018).